Department of Medical Biology and Genetics, Medical Faculty, Istanbul Aydin University, Istanbul, Turkey.
Department of Medical Biology, Medical Faculty, Eskisehir Osmangazi University, Eskisehir, Turkey.
Hum Exp Toxicol. 2019 Nov;38(11):1296-1301. doi: 10.1177/0960327119864154. Epub 2019 Jul 17.
The aim of the study was to determine the relationship between anti-inflammatory effects of the natural polyphenolic compound tannic acid (CAS number: 1401-55-4) and myeloperoxidase (MPO) enzyme activity in paw edema model. Thirty-five female rats were divided into five groups. The paws of rats were injected subcutaneously in the plantar surface with formalin except for the control group. Indomethacin and tannic acid were intraperitoneally administered 1 h after formalin injection. The paws volume was measured by using vernier caliper. MPO enzyme activity was determined using 4-aminoantipyrine-phenol solution as the substrate for MPO-mediated oxidation by HO. About 17% and 13% edema inhibition has detected in the indomethacin-applied group, at the measurements run every other hour right after the treatment. An inhibition of 16% was found at the group treated with 25 mg/kg tannic acid. However, in the group treated with 50 mg/kg tannic acid, 15% and 7% of the edema inhibition was observed. Serum and paw tissue MPO activities were decreased in treated groups with indomethacin and tannic acid according to formalin control group. Our study results suggest that tannic acid may contribute to the treatment of inflammation by decreasing MPO enzyme activity, but the molecular mechanism is still not clear.
本研究旨在确定天然多酚化合物单宁酸（CAS 号：1401-55-4）的抗炎作用与爪肿胀模型中髓过氧化物酶（MPO）酶活性之间的关系。35 只雌性大鼠被分为五组。除对照组外，向大鼠足底皮下注射福尔马林。福尔马林注射后 1 小时，通过腹腔内给予消炎痛和单宁酸。使用游标卡尺测量爪子的体积。使用 4-氨基安替比林-苯酚溶液作为 MPO 介导的 HO 氧化的底物来测定 MPO 酶活性。在治疗后每隔一小时进行的测量中，在应用消炎痛的组中检测到约 17%和 13%的水肿抑制。在应用 25mg/kg 单宁酸的组中发现 16%的抑制。然而，在应用 50mg/kg 单宁酸的组中，观察到 15%和 7%的水肿抑制。根据福尔马林对照组，应用消炎痛和单宁酸的治疗组血清和爪组织 MPO 活性降低。我们的研究结果表明，单宁酸可能通过降低 MPO 酶活性来促进炎症的治疗，但分子机制尚不清楚。
