Wunnoo Suttiwan, Sermwittayawong Decha, Praparatana Rachanida, Voravuthikunchai Supayang Piyawan, Jakkawanpitak Chanawee
Center of Antimicrobial Biomaterial Innovation-Southeast Asia, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
Center of Excellence for Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
Antioxidants (Basel). 2024 Sep 9;13(9):1094. doi: 10.3390/antiox13091094.
Olivier (Fagaceae) nutgall, a traditional Asian medicine, is renowned for its efficacy in treating wounds and skin disorders. Although the gall extract has shown promising results in accelerating wound healing in diabetic animal models, its mechanisms, particularly the effects on redox balance, remain poorly understood. This study aims to investigate the effects and mechanisms of gall ethanolic extract (QIG) on wound healing in fibroblasts, with a specific emphasis on its modulation of oxidative stress. Hydrogen peroxide (HO)-treated L929 cells were used as an in vitro model of oxidation-damaged fibroblasts. QIG exhibited potent antioxidant activity with 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assay values of 305.43 ± 7.48, 508.94 ± 15.12, and 442.08 ± 9.41 µM Trolox equivalents (TE)/µg, respectively. Elevated HO levels significantly reduced L929 cell viability, with a 50% lethal concentration of 1.03 mM. QIG mitigated HO-induced cytotoxicity in a dose-dependent manner, showing protective effects in pre-, post-, and co-treatment scenarios. QIG significantly reduced HO-induced intracellular reactive oxygen species production and inflammation-related gene expression ( < 0.05). Additionally, at 25 µg/mL, QIG remarkably improved wound closure in HO-treated L929 cells by approximately 9.4 times compared with the HO treatment alone ( < 0.05). These findings suggest QIG has potential therapeutic applications in wound healing, mediated through the regulation of oxidative stress and inflammatory response.
橡子（壳斗科）五倍子是一种传统的亚洲药物，以其治疗伤口和皮肤疾病的功效而闻名。尽管五倍子提取物在加速糖尿病动物模型伤口愈合方面已显示出有前景的结果，但其机制，特别是对氧化还原平衡的影响，仍知之甚少。本研究旨在探讨五倍子乙醇提取物（QIG）对成纤维细胞伤口愈合的影响及机制，特别强调其对氧化应激的调节作用。用过氧化氢（H₂O₂）处理的L929细胞作为氧化损伤成纤维细胞的体外模型。QIG在2,2-二苯基-1-苦基肼（DPPH）、2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）和铁还原抗氧化能力（FRAP）测定中表现出强大的抗氧化活性，其值分别为305.43±7.48、508.94±15.12和442.08±9.41μM Trolox当量（TE）/μg。升高的H₂O₂水平显著降低了L929细胞活力，50%致死浓度为1.03 mM。QIG以剂量依赖的方式减轻H₂O₂诱导的细胞毒性，在预处理、后处理和共处理情况下均显示出保护作用。QIG显著降低了H₂O₂诱导的细胞内活性氧产生和炎症相关基因表达（P<0.05）。此外，在25μg/mL时，与单独的H₂O₂处理相比，QIG使H₂O₂处理的L929细胞中的伤口闭合显著改善了约9.4倍（P<0.05）。这些发现表明，QIG通过调节氧化应激和炎症反应在伤口愈合中具有潜在的治疗应用。
