Kurbakov Konstantin A, Konorov Evgenii A, Minaev Mikhail Y, Kuznetsova Oksana A
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Talalikhina 26, 109316, Moscow, Russian Federation.
Vavilov Institute of General Genetics, Gubkina 3, 119333, Moscow, Russian Federation.
Food Technol Biotechnol. 2019 Mar;57(1):97-104. doi: 10.17113/ftb.57.01.19.5983.
Optimization of fermentation processes requires monitoring the species composition of starter cultures and their growth during fermentation. Most starter cultures contain closely related species. Nowadays, high-resolution melting (HRM) analysis is extensively used for multiplex identification of closely related species. In the present paper, we applied real-time polymerase chain reaction (PCR) with HRM analysis for the detection and differentiation of and . A primer pair was selected for the site of the gene of spp. Eleven starter cultures and fifteen fermented sausages with a known bacterial composition were successfully tested using real-time PCR with HRM analysis with the developed primer pair.
发酵过程的优化需要监测发酵剂培养物的物种组成及其在发酵过程中的生长情况。大多数发酵剂培养物包含密切相关的物种。如今，高分辨率熔解（HRM）分析被广泛用于密切相关物种的多重鉴定。在本文中，我们应用实时聚合酶链反应（PCR）结合HRM分析来检测和区分[具体物种]。针对[具体物种]的[具体基因]位点选择了一对引物。使用所开发的引物对，通过实时PCR结合HRM分析，成功地对11种发酵剂培养物和15种已知细菌组成的发酵香肠进行了检测。
