Tani Y, Sugiyama H, Maeda T, Miyake S, Oka Y, Ogawa H, Komori T, Soma T, Kishimoto S
Third Department of International Medicine, Osaka University Medical School, Japan.
Mol Immunol. 1988 Feb;25(2):127-36. doi: 10.1016/0161-5890(88)90060-0.
Class switching from gamma 3 to gamma 2b production at pre-B cell stage was observed in an Abelson virus-transformed murine immature cell line that is able to perform VH to DJH recombinations followed by class switch recombinations. A series of Southern blotting experiments indicates that the class switching from gamma 3 to gamma 2b was mediated by the deletion mechanism of the intervening CH genes on the expressed chromosome and that the break points in the switch recombinations might be different from each other among six independent switching events from gamma 3 to gamma 2b. This in vitro switching system should provide us with much information on the mechanisms of class switch recombination.
在一种能够进行VH到DJH重组继而进行类别转换重组的艾贝尔森病毒转化的小鼠未成熟细胞系中，观察到了前B细胞阶段从γ3到γ2b产生的类别转换。一系列Southern印迹实验表明，从γ3到γ2b的类别转换是由表达染色体上中间CH基因的缺失机制介导的，并且在从γ3到γ2b的六个独立转换事件中，转换重组中的断点可能彼此不同。这种体外转换系统应该为我们提供许多关于类别转换重组机制的信息。
