Ali Huda R K, Hemeda Nada F, Abdelaliem Yasser F
Plant Protection Research Institute, A. R. C, Giza, Egypt.
Department of Genetics, Faculty of Agriculture, Fayoum University, El Faiyûm, Egypt.
AMB Express. 2019 Jul 17;9(1):111. doi: 10.1186/s13568-019-0830-5.
The subterranean termite Psammotermes hypostoma Desneux is considered as an important pest that could cause severe damage to buildings, furniture, silos of grain and crops or any material containing cellulose. This species of termites is widespread in Egypt and Africa. The lower termite's ability to digest cellulose depends on the association of symbiotic organisms gut that digest cellulose (flagellates and bacteria). In this study, 33 different bacterial isolates were obtained from the gut of the termite P. hypostoma which were collected using cellulose traps. Strains were grown on carboxymethylcellulose (CMC) as a sole source of carbon. Cellulolytic strains were isolated in two different cellulose medium (mineral salt medium containing carboxymethylcellulose as the sole carbon source and agar cellulose medium). Five isolates showed significant cellulolytic activity identified by a Congo red assay which gives clear zone. Based on biochemical tests and sequencing of 16s rRNA genes these isolates were identified as Paenibacillus lactis, Lysinibacillus macrolides, Stenotrophomonas maltophilia, Lysinibacillus fusiformis and Bacillus cereus, that deposited in GenBank with accession numbers MG991563, MG991564, MG991565, MG991566 and MG991567, respectively.
地下白蚁哈氏象白蚁（Psammotermes hypostoma Desneux）被认为是一种重要害虫，会对建筑物、家具、谷物和农作物筒仓或任何含纤维素的材料造成严重损害。这种白蚁在埃及和非洲广泛分布。低等白蚁消化纤维素的能力取决于其肠道中消化纤维素的共生生物（鞭毛虫和细菌）的联合作用。在本研究中，从哈氏象白蚁肠道中获得了33种不同的细菌分离株，这些分离株是使用纤维素诱捕器收集的。菌株在羧甲基纤维素（CMC）作为唯一碳源的培养基上生长。在两种不同的纤维素培养基（以羧甲基纤维素作为唯一碳源的无机盐培养基和琼脂纤维素培养基）中分离出纤维素分解菌株。通过刚果红试验鉴定出5种具有显著纤维素分解活性的分离株，该试验会产生清晰的透明圈。基于生化试验和16s rRNA基因测序，这些分离株被鉴定为乳酸芽孢杆菌（Paenibacillus lactis）、大环赖氨酸芽孢杆菌（Lysinibacillus macrolides）、嗜麦芽窄食单胞菌（Stenotrophomonas maltophilia）、梭形赖氨酸芽孢杆菌（Lysinibacillus fusiformis）和蜡样芽孢杆菌（Bacillus cereus），它们分别以登录号MG991563、MG991564、MG991565、MG991566和MG991567保存在GenBank中。
