Department of Plant Protection, Faculty of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Plant Protection, College of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Curr Microbiol. 2021 Apr;78(4):1305-1316. doi: 10.1007/s00284-021-02369-0. Epub 2021 Feb 27.
Termites are a large and important group of insects in terrestrial ecosystems that decompose lignocelluloses. Among these, Microcerotermes diversus Silvestri (Termitidae) is a destructive invasive pest in many tropical and subtropical regions. In the present study, M. diversus specimens were collected from traps in Ahvaz, Bandar Abbas, Kish, and Khark Islands. Sample suspensions were prepared in 5-ml DHO and cultured on nutrient agar (NA) medium. All 47 representative bacterial isolates were evaluated for cellulolytic activity by growing them on cellulose Congo-red agar medium. Based on some key phenotypic characteristics, the isolates were tentatively identified at the genus level. These were confirmed by 16S rRNA analysis using a universal primer pair (P1/P6). Sequence alignments revealed that most of the isolates are novel species so far found in the termite guts. Results showed that some of the isolates are common for all surveyed areas. However, there were significant differences in their numbers and degree of cellulolytic activity. The species reported here for the first time for termites of Iran are Bacillus wiedmanii, B. paramycoides, Elizabethkingia anophelis, Lysinibacillus pakestanensis, Pseudomonas hibiscicola, Actinetobacter pitti, A. venetianus, and Ochrobactrum anthropi.
白蚁是陆地生态系统中一个庞大而重要的昆虫类群，它们能分解木质纤维素。其中，异沙白蚁（Termitidae）是许多热带和亚热带地区具有破坏性的入侵害虫。本研究从阿瓦士、班达尔阿巴斯、基什和哈尔克岛的诱捕器中采集了异沙白蚁标本。将样品悬浮液制备在 5ml 的 DHO 中，并在营养琼脂（NA）培养基上培养。通过在纤维素刚果红琼脂培养基上培养所有 47 个具有代表性的细菌分离物来评估其纤维素酶活性。根据一些关键的表型特征，将分离物暂定鉴定为属水平。通过使用通用引物对（P1/P6）进行 16S rRNA 分析来确认这些分类。序列比对显示，大多数分离物是迄今为止在白蚁肠道中发现的新物种。结果表明，一些分离物在所有调查区域都很常见。然而，它们的数量和纤维素酶活性存在显著差异。这里报道的一些物种是伊朗白蚁的首次报道，包括韦氏芽孢杆菌、类芽孢杆菌、嗜人按蚊伊丽莎白菌、巴基斯坦盐单胞菌、美丽假单胞菌、不动杆菌、威尼斯不动杆菌和奥克霍姆氏菌。
