Department of Pharmacology, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.
College of Medicine and Health Sciences, University of Rwanda, Kigali, Rwanda.
HIV Med. 2019 Aug;20(7):485-495. doi: 10.1111/hiv.12782.
In this study, we determined the incidence and persistence of human papillomavirus (HPV) strains and of squamous intraepithelial lesions (SIL) or worse cytology in 237 HIV-positive and HIV-negative Rwandan women and whether the interleukin (IL)-28B single nucleotide polymorphism (SNP) at rs12979860 correlated with susceptibility to and persistence of HPV infection.
Cervical samples were collected at baseline and after 9, 18 and 24 months for a 40-HPV DNA screening test and a ThinPrep Pap test. Genotyping of the IL-28B SNP rs12979860 was performed using real-time polymerase chain reaction (PCR).
Chronic high-risk (HR) HPV infections occurred in 56% of HIV-positive women, while no HIV-negative women developed HPV chronicity. High-grade SIL (HSIL) or cancer was diagnosed in 38% of HIV-positive women with persistent HR-HPV infections. HIV and HR-HPV positivity at baseline were factors associated with an increased risk of HPV persistence. Additionally, HR-HPV positivity at baseline was associated with an increased risk of developing HSIL or worse cytology. The unfavourable T/x genotype at rs12979860 is common among Africans, and women with this genotype were found to be more commonly infected with HPV.
HPV screening in Rwanda may help to identify women at risk of developing cervical cancer and polymorphism in IL-28B may be associated with risk of contracting HPV infection.
在这项研究中，我们确定了在 237 名卢旺达 HIV 阳性和 HIV 阴性妇女中，人乳头瘤病毒（HPV）株以及鳞状上皮内病变（SIL）或更差细胞学的发生率和持续性，以及白细胞介素（IL）-28B 单核苷酸多态性（SNP）rs12979860 是否与 HPV 感染的易感性和持续性相关。
在基线时和 9、18 和 24 个月时收集宫颈样本，用于进行 40 种 HPV DNA 筛查试验和 ThinPrep Pap 试验。使用实时聚合酶链反应（PCR）对 IL-28B SNP rs12979860 进行基因分型。
慢性高危（HR）HPV 感染发生在 56%的 HIV 阳性妇女中，而没有 HIV 阴性妇女发生 HPV 慢性感染。持续性 HR-HPV 感染的 HIV 阳性妇女中有 38%诊断为高级别 SIL（HSIL）或癌症。基线时的 HIV 和 HR-HPV 阳性是 HPV 持续感染风险增加的相关因素。此外，基线时的 HR-HPV 阳性与发生 HSIL 或更差细胞学的风险增加相关。rs12979860 处不利的 T/x 基因型在非洲人中很常见，携带这种基因型的妇女更常感染 HPV。
在卢旺达进行 HPV 筛查可能有助于识别有发展宫颈癌风险的妇女，而 IL-28B 多态性可能与 HPV 感染的风险相关。
