Department of Chemistry and Skaggs Institute for Chemical Biology , The Scripps Research Institute , 10550 N. Torrey Pines Road , La Jolla , California 92037 , United States.
Department of Chemistry and Chemical Biology , Rutgers, The State University of New Jersey , Piscataway , New Jersey 08854 , United States.
Bioconjug Chem. 2019 Aug 21;30(8):2102-2105. doi: 10.1021/acs.bioconjchem.9b00413. Epub 2019 Jul 23.
We have genetically encoded a dithiolane containing amino acid (dtF) in () using a polyspecific aminoacyl-tRNA synthetase (aaRS)/amber suppressor tRNA pair. To demonstrate the utility of dtF for bioapplications, we synthesized gold nanoparticle (AuNP) constructs with a mutant superfolder green fluorescent protein (sfGFP) [sfGFP-AuNP] as a model for the protein-metal conjugation. The resulting sfGFP-AuNP constructs show directional homogeneity and enhanced chemical durability compared to their cysteine analogues toward excess environmental 1,4-dithiothreitol (DTT).
我们使用多特异性氨酰-tRNA 合成酶（aaRS）/琥珀终止 tRNA 对，在 () 中遗传编码了一种含二硫戊环的氨基酸（dtF）。为了展示 dtF 在生物应用中的实用性，我们以突变型超折叠绿色荧光蛋白（sfGFP）[sfGFP-AuNP]为模型，合成了金纳米颗粒（AuNP）构建体，用于蛋白质-金属偶联。与巯基乙烷硫醇（DTT）相比，所得 sfGFP-AuNP 构建体在方向均匀性和增强的化学稳定性方面优于其半胱氨酸类似物。
