一种正交的丝氨酰-tRNA 合成酶/ tRNA 对用于大肠杆菌中非规范氨基酸诱变。
An orthogonal seryl-tRNA synthetase/tRNA pair for noncanonical amino acid mutagenesis in Escherichia coli.
机构信息
Department of Chemistry and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, United States.
Departments of Biological Sciences and Chemistry, Bridge Institute, Michelson Center for Convergent Bioscience, University of Southern California, Los Angeles, CA 90089, United States.
出版信息
Bioorg Med Chem. 2020 Oct 15;28(20):115662. doi: 10.1016/j.bmc.2020.115662. Epub 2020 Jul 28.
Abstract
We report the development of the orthogonal amber-suppressor pair Archaeoglobus fulgidus seryl-tRNA (Af-tRNA)/Methanosarcina mazei seryl-tRNA synthetase (MmSerRS) in Escherichia coli. Furthermore, the crystal structure of MmSerRS was solved at 1.45 Å resolution, which should enable structure-guided engineering of its active site to genetically encode small, polar noncanonical amino acids (ncAAs).
摘要
我们报告了古菌门火球菌丙氨酰-tRNA（Af-tRNA）/产甲烷八叠球菌丙氨酰-tRNA 合成酶（MmSerRS）正交琥珀终止密码子对在大肠杆菌中的开发。此外，MmSerRS 的晶体结构已解析至 1.45Å 分辨率，这应该能够通过结构指导工程改造其活性位点，从而实现对小的、极性非典型氨基酸（ncAA）的遗传编码。
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