CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477, Lisboa, Portugal; Laboratório de Virologia, Instituto Nacional de Investigação Agrária e Veterinária (INIAV), Quinta do Marquês, 2780-157, Oeiras, Portugal.
CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477, Lisboa, Portugal.
Virus Res. 2019 Sep;270:197667. doi: 10.1016/j.virusres.2019.197667. Epub 2019 Jul 15.
African swine fever (ASF) is currently matter for major concerns in global swine industry as it is highly contagious and causes acute fatal haemorrhagic fever in domestic pigs and wild boar. The absence of effective vaccines and treatments pushes ASF control to relay on strict sanitary and stamping out measures with costly socio-economic impacts. The current epidemic scenario of fast spreading throughout Asiatic countries impels further studies on prevention and combat strategies against ASF. Herein we review knowledge on African Swine Fever Virus (ASFV) interactions with the host cell nucleus and on the functional properties of different viral DNA-replication related proteins. This entails, the confirmation of an intranuclear viral DNA replication phase, the characterization of cellular DNA damage responses (DDR), the subnuclear compartments disruption due to viral modulation, and the unravelling of the biological role of several viral proteins (A104R, I215 L, P1192R, QP509 L and Q706 L), so to contribute to underpin rational strategies for vaccine candidates development.
非洲猪瘟（ASF）是目前全球养猪业的主要关注点，因为它具有高度传染性，会导致家猪和野猪急性致命性出血热。由于缺乏有效的疫苗和治疗方法，ASF 的防控措施只能依靠严格的卫生和扑灭措施，这会带来高昂的社会经济影响。ASF 在亚洲国家迅速传播的现状促使人们进一步研究预防和对抗 ASF 的策略。本文综述了非洲猪瘟病毒（ASFV）与宿主细胞核相互作用以及不同病毒 DNA 复制相关蛋白的功能特性的研究进展。这包括确认病毒 DNA 复制的核内阶段，鉴定细胞 DNA 损伤反应（DDR），病毒调节导致亚核区室破坏，以及揭示几种病毒蛋白（A104R、I215L、P1192R、QP509L 和 Q706L）的生物学作用，从而为疫苗候选物的开发提供合理策略的基础。
