Plant Health Department, NEIKER-Tecnalia, Arkaute, Spain; iBB - Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
iBB - Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
Bioresour Technol. 2019 Oct;290:121785. doi: 10.1016/j.biortech.2019.121785. Epub 2019 Jul 10.
The organic fraction of municipal solid waste was studied as feedstock for the production of poly(3-hydroxybutyrate) (P(3HB)). To release the monosaccharides, a diluted acid pre-treatment followed by an enzymatic hydrolysis was applied. A sugar yield of 49% was achieved using a pre-treated waste and an enzyme cocktail of Pentopan 500 BG and Celluclast BG. The addition of Glucoamylase NS 22035 helped to hydrolyze the starch fraction, improving the hydrolysis yield to 56%. The hydrolysate was used as culture medium to produce P(3HB) by Burkholderia sacchari DSM 17165. Assays at shaking flask scale showed that when the hydrolysate was used as substrate, the attained cell concentration was slightly higher than in the control medium. It was necessary to supplement the hydrolysate with extra glucose to increase the C/N ratio and with a mineral solution to overcome the nutritional deficiencies. The P(3HB) accumulation using the supplemented hydrolysate was 58% (g polymer/g biomass).
研究了城市固体废物的有机部分作为生产聚（3-羟基丁酸酯）（P(3HB)）的原料。为了释放单糖，采用了稀酸预处理，然后进行酶水解。使用预处理废物和 Pentopan 500 BG 和 Celluclast BG 的酶混合物，可实现 49%的糖产率。添加 Glucoamylase NS 22035 有助于水解淀粉部分，将水解产率提高到 56%。将水解物用作培养基于 Burkholderia sacchari DSM 17165 生产 P(3HB)。摇瓶试验表明，当使用水解物作为底物时，获得的细胞浓度略高于对照培养基。有必要用额外的葡萄糖补充水解物，以增加 C/N 比，并使用矿物溶液克服营养不足。使用补充水解物的 P(3HB)积累量为 58%（g 聚合物/g 生物质）。
