Laboratory of Gastrointestinal Microbiology, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing 210095, China.
Laboratory of Gastrointestinal Microbiology, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing 210095, China.
Bioresour Technol. 2019 Oct;290:121796. doi: 10.1016/j.biortech.2019.121796. Epub 2019 Jul 12.
The aim of this study was to investigate the effects of steam explosion on lignocellulose digestibility of, and methane production from corn stover by a co-culture of anaerobic fungus and methanogen. The cumulative methane production at 72 h of incubation from the steam-exploded corn stover was 32.2 ± 1.74 mL, which not significantly different (P > 0.05) from that of the untreated corn stover (37.1 ± 1.09 mL). However, steam explosion decreased the hemicellulose contents of corn stover by 28.0 ± 0.39% and increased the neutral detergent solute by 23.5 ± 0.25%. While this treatment did not affect the dry matter digestibility (64.1 ± 0.26%, and 64.1 ± 0.28%, respectively). In conclusion, the co-culture of anaerobic fungus and methanogen can degrade the crude fibrous portion of corn stover without any pretreatments. It possesses promising biotechnological prospects for conversion of crop residue based straw resources to obtain biofuel in the form of methane.
本研究旨在通过厌氧真菌和产甲烷菌的共培养来研究蒸汽爆破对玉米秸秆木质纤维素消化率和甲烷产量的影响。在 72 h 孵育期内，蒸汽爆破玉米秸秆的累积甲烷产量为 32.2±1.74 mL，与未经处理的玉米秸秆（37.1±1.09 mL）的甲烷产量无显著差异（P＞0.05）。然而，蒸汽爆破使玉米秸秆的半纤维素含量降低了 28.0±0.39%，而中性洗涤剂溶质增加了 23.5±0.25%。虽然这一处理并没有影响干物质消化率（分别为 64.1±0.26%和 64.1±0.28%）。综上所述，厌氧真菌和产甲烷菌的共培养可以在不进行任何预处理的情况下降解玉米秸秆的粗纤维部分。它在利用作物残余物基秸秆资源以甲烷的形式获得生物燃料方面具有广阔的生物技术前景。
