Li Yuqi, Hou Zhesheng, Shi Qicheng, Cheng Yanfen, Zhu Weiyun
Laboratory of Gastrointestinal Microbiology, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, China.
Joint International Research Laboratory of Animal Health and Food Safety, Nanjing Agriculture University, Nanjing, China.
Front Bioeng Biotechnol. 2020 Apr 30;8:314. doi: 10.3389/fbioe.2020.00314. eCollection 2020.
To determine ways to improve the utilization of corn stover, this study investigated methane production from different parts of corn stover using a simple co-culture of an anaerobic fungus ( species) and methanogen ( species). The simple co-culture was incubated with the stem pith, leaf blade, or stem bark of corn stover (as substrates) at 39°C for 72 h. The results showed that the stem bark had the lowest ( < 0.05) digestibility (38.0 ± 1.36%) and neutral detergent solubles, that is, cell solubles (31.6 ± 0.45%), and the highest ( < 0.05) lignin content (4.8 ± 0.56%). The leaf blade had a significantly higher methane conversion rate (56.6 ± 0.76 mL/g digested substrate) than the stem pith (49.2 ± 1.60 mL/g digested substrate), even though they showed similar levels of methane production (42.4 ± 1.0 mL and 40.9 ± 1.35 mL, respectively). Both the leaf blade and stem pith of corn stover have the potential to produce methane in a simple co-culture of an anaerobic fungus and methanogen.
为了确定提高玉米秸秆利用率的方法，本研究使用厌氧真菌（种）和产甲烷菌（种）的简单共培养体系，研究了玉米秸秆不同部位的甲烷产生情况。将该简单共培养体系与玉米秸秆的茎髓、叶片或茎皮（作为底物）在39℃下孵育72小时。结果表明，茎皮的消化率最低（P<0.05）（38.0±1.36%），中性洗涤剂可溶物即细胞可溶物含量也最低（31.6±0.45%），而木质素含量最高（P<0.05）（4.8±0.56%）。叶片的甲烷转化率（56.6±0.76 mL/g消化底物）显著高于茎髓（49.2±1.60 mL/g消化底物），尽管它们的产甲烷量相似（分别为42.4±1.0 mL和40.9±1.35 mL）。玉米秸秆的叶片和茎髓在厌氧真菌和产甲烷菌的简单共培养体系中都有产生甲烷的潜力。
