School of Marine Science and Technology, Harbin Institute of Technology, Weihai, 2(#) Wenhua West Road, Weihai 264209, PR China; State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, PR China.
School of Marine Science and Technology, Harbin Institute of Technology, Weihai, 2(#) Wenhua West Road, Weihai 264209, PR China; Guangzhou Key Laboratory of Environmental Exposure and Health, School of Environment, Jinan University, Guangzhou 510632, PR China.
Environ Pollut. 2019 Oct;253:239-245. doi: 10.1016/j.envpol.2019.07.014. Epub 2019 Jul 8.
In the study, the effects of dimethyl phthalate (DMP) on the antioxidant defense capacity and immune functions of human erythrocytes were experimentally explored. DMP affected the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) and the contents of glutathione (GSH) and malondialdehyde (MDA) in erythrocytes, thus impairing the function of antioxidant defense system of erythrocytes. When DMP concentration increased from 0 to 28 μmol L, the SOD and GPX activities were increased firstly and then gradually decreased. When DMP concentration was below 20 μmol L, the relative activity of SOD was enhanced by DMP and the effect was known as hormesis. The relative activity of GPX was also increased when the concentration of DMP was below 12 μmol L. The CAT activity was more significantly inhibited by DMP than the activities of SOD and GPX, whereas the relative GSH content was increased by DMP. MDA levels were significantly changed after the exposure to DMP (0-24 μmol L). The experimental results of the activity of SOD and CAT, and the content of MDA also suggested that DMP could inhibit the immune functions of red blood cells (RBCs), which were further proved by the decrease of two indicators (RBC-Cb and RBC-IC) due to the destruction of Cb receptor with immune adherence function on erythrocyte membrane. The study provides a deep understanding of the toxicity of DMP on erythrocytes.
在这项研究中，实验性地探讨了邻苯二甲酸二甲酯（DMP）对人红细胞抗氧化防御能力和免疫功能的影响。DMP 影响超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）的活性以及红细胞中谷胱甘肽（GSH）和丙二醛（MDA）的含量，从而损害了红细胞抗氧化防御系统的功能。当 DMP 浓度从 0 增加到 28 μmol/L 时，SOD 和 GPX 的活性先增加后逐渐降低。当 DMP 浓度低于 20 μmol/L 时，DMP 增强了 SOD 的相对活性，这种效应被称为激素作用。当 DMP 浓度低于 12 μmol/L 时，GPX 的相对活性也增加。CAT 活性比 SOD 和 GPX 的活性受 DMP 的抑制更为显著，而 DMP 增加了相对 GSH 含量。暴露于 DMP（0-24 μmol/L）后 MDA 水平发生显著变化。SOD 和 CAT 活性以及 MDA 含量的实验结果也表明，DMP 可以抑制红细胞（RBC）的免疫功能，这进一步通过 Cb 受体的破坏得到证明，该受体在红细胞膜上具有免疫粘附功能。该研究深入了解了 DMP 对红细胞的毒性。
