Miyamoto M, Sasakawa S
Central Blood Center, Japanese Red Cross, Tokyo.
Vox Sang. 1988;54(2):74-7. doi: 10.1111/j.1423-0410.1988.tb01620.x.
Autoclave sterilization altered the leaching of plasticizer, CO2 gas permeability, surface area and the surface wettability of bag films. These changes affected granulocyte cell counts and functions during storage. Four types of polyvinyl chloride bags, with di-(2-ethylhexyl)phthalate (DEHP) or tri-(2-ethylhexyl)trimellitate (TOTM) as plasticizer, with or without treatment by glow discharge (H2), were sterilized with ethylene oxide (EO) or autoclaving (AC). The greatest amounts of plasticizer leached from DEHP-EO bags. TOTM plasticizer did not leach into plasma. CO2 gas permeability was greater with TOTM than DEHP. AC sterilization decreased the surface area of bags. Wettability of film surfaces was greatest with H2-TOTM-EO. After storage in these bags for 24 and 48 h at 22 degrees C, the granulocyte cell counts and functions were greatest in H2-TOTM-EO bags with the nonleaching plasticizer, higher CO2 gas permeability and higher wettable surface due to glow-discharge treatment. The H2-TOTM-EO bag was useful as a granulocyte storage container.
高压蒸汽灭菌改变了增塑剂的浸出、二氧化碳气体渗透性、表面积以及袋膜的表面润湿性。这些变化影响了储存期间粒细胞的细胞计数和功能。四种以邻苯二甲酸二（2-乙基己基）酯（DEHP）或偏苯三酸三（2-乙基己基）酯（TOTM）作为增塑剂的聚氯乙烯袋，有或没有经过辉光放电（H2）处理，分别用环氧乙烷（EO）或高压蒸汽灭菌（AC）进行灭菌。从DEHP-EO袋中浸出的增塑剂最多。TOTM增塑剂不会渗入血浆。TOTM的二氧化碳气体渗透性比DEHP大。高压蒸汽灭菌降低了袋子的表面积。膜表面的润湿性在H2-TOTM-EO袋中最大。在22摄氏度下于这些袋子中储存24小时和48小时后，由于辉光放电处理，具有不浸出增塑剂、较高二氧化碳气体渗透性和较高可润湿性表面的H2-TOTM-EO袋中的粒细胞细胞计数和功能最佳。H2-TOTM-EO袋可用作粒细胞储存容器。
