School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China; Wenzhou Institute of Biomaterials and Engineering, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang 325011, China.
Wenzhou Institute of Biomaterials and Engineering, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang 325011, China.
Carbohydr Polym. 2019 Oct 15;222:115012. doi: 10.1016/j.carbpol.2019.115012. Epub 2019 Jun 20.
It is important to control immediate hemorrhage and prevent infection simultaneously in the wound management. However, most of hemostatic materials are associated with low efficiency of hemostasis, poor biocompatibility and lack of antimicrobial properties. A kind of starch-based macroporous sponges (KR-Sps) immobilized covalently with antimicrobial peptide KR12 using highly efficient thiol-ene photo click reaction were developed. The physical properties of these sponges could be fine-tuned by varying the ratio of modified starch/HS-PEG-SH and the polymer concentration. The in vitro and vivo results demonstrated that KR-Sps induced thrombosis, shortened clotting time and reduced the blood loss at bleeding site. Besides, KR12 immobilized sponge exhibited inherent antimicrobial properties against Gram (+) and (-) bacteria and methicillin-resistant Staphylococcus aureus (MRSA), which could maintain at least 5 days. Therefore, KR-Sps were believed to be an excellent candidate as hemostatic and antimicrobial product for the intraoperative wound management.
在伤口管理中，控制即时出血和预防感染同样重要。然而，大多数止血材料的止血效率低、生物相容性差且缺乏抗菌性能。本研究采用高效的硫醇-烯点击反应，将具有抗菌肽 KR12 的淀粉基大孔海绵（KR-Sps）通过共价键固定。这些海绵的物理性质可以通过改变改性淀粉/HS-PEG-SH 的比例和聚合物浓度来进行微调。体外和体内结果表明，KR-Sps 可诱导血栓形成，缩短凝血时间，并减少出血部位的出血量。此外，固定有 KR12 的海绵对革兰氏阳性菌和革兰氏阴性菌以及耐甲氧西林金黄色葡萄球菌（MRSA）具有固有抗菌性能，至少可维持 5 天。因此，KR-Sps 有望成为一种优秀的止血和抗菌产品，可用于术中伤口管理。
