Hirabayashi Miyuki, Chambers James K, Sugawara Mei, Ohmi Aki, Tsujimoto Hajime, Nakayama Hiroyuki, Uchida Kazuyuki
Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, Japan.
Veterinary Medical Center, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, Japan.
JFMS Open Rep. 2019 Jul 11;5(2):2055116919863080. doi: 10.1177/2055116919863080. eCollection 2019 Jul-Dec.
A 7-year-old mixed-breed cat presented with subcutaneous oedema and erythema extending from the right axilla to the abdomen. Fine-needle aspiration of the subcutaneous lesion revealed large, atypical, round cells. A clonality analysis for the T-cell receptor-gamma and immunoglobulin heavy chain genes showed no clonal rearrangement. The presumed diagnosis was lymphoma and the cat was treated with prednisolone and L-asparaginase but died 78 days after initial treatment. At necropsy, an oedematous subcutaneous mass in the right axilla, hepatomegaly, splenomegaly and lymphadenopathy of the mediastinum and left axilla were observed. Histopathological examination revealed diffuse infiltration of large atypical round cells in the subcutaneous mass, liver, spleen, lymph nodes and bone marrow. Immunohistochemically, the tumour cells were strongly positive for CD56, and negative for CD3, CD20, CD79a, CD57, granzyme B and perforin. Based on these findings, the cat was diagnosed with blastic natural killer (NK) cell lymphoma/leukaemia.
Here, we report the pathological and clinical findings of NK cell lymphoma/leukaemia in a cat. The antibody for human CD56, a diagnostic marker for human NK cell neoplasms, showed cross-reactivity with feline CD56 by immunohistochemistry and Western blotting analysis. The antibody could be a useful diagnostic marker for feline NK cell neoplasms.
一只7岁的混种猫出现皮下水肿和红斑，从右腋窝延伸至腹部。对皮下病变进行细针穿刺活检，发现有大的、非典型的圆形细胞。对T细胞受体γ基因和免疫球蛋白重链基因进行克隆性分析，结果显示无克隆重排。初步诊断为淋巴瘤，该猫接受泼尼松龙和L-天冬酰胺酶治疗，但在初始治疗78天后死亡。尸检时，发现右腋窝有一个水肿性皮下肿块，肝脏肿大、脾脏肿大以及纵隔和左腋窝淋巴结病。组织病理学检查显示，皮下肿块、肝脏、脾脏、淋巴结和骨髓中有大量非典型圆形细胞弥漫浸润。免疫组织化学检查显示，肿瘤细胞CD56呈强阳性，而CD3、CD20、CD79a、CD57、颗粒酶B和穿孔素呈阴性。基于这些发现，该猫被诊断为原始自然杀伤（NK）细胞淋巴瘤/白血病。
在此，我们报告了一只猫的NK细胞淋巴瘤/白血病的病理和临床发现。人CD56抗体是人类NK细胞肿瘤的诊断标志物，通过免疫组织化学和蛋白质印迹分析显示，该抗体与猫CD56有交叉反应性。该抗体可能是猫NK细胞肿瘤的一种有用的诊断标志物。
