Ii Tatsuhito, Chambers James K, Segawa Kazuhito, Uchida Kazuyuki
Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Sagamihara Animal Medical Centre, Sagamihara, Kanagawa, Japan.
JFMS Open Rep. 2021 Apr 20;7(1):20551169211005914. doi: 10.1177/20551169211005914. eCollection 2021 Jan-Jun.
A 14-year 3-month-old spayed female mixed-breed cat presented with jaundice, anaemia and thrombocytopenia. Haemophagocytic syndrome associated with lymphoma was suspected after cytological examination of the spleen. Despite treatment with prednisolone, L-asparaginase and nimustine, the cat died 176 days after the initial presentation. Necropsy revealed splenomegaly and hepatomegaly, without lymphadenopathy. Histopathologically, neoplastic lymphoid cells infiltrated the hepatic sinusoid and splenic sinus. The neoplastic lymphoid cells showed marked hepatocytotropism and contained erythrocytes, which was also confirmed by electron microscopy. Immunohistochemically, neoplastic lymphoid cells were positive for CD3, TIA1 (GMP-17) and granzyme B, and negative for CD8, CD20, CD56, CD57, CD79a and Iba1. Based on these findings, the cat was diagnosed with hepatosplenic T-cell lymphoma (HS-TCL) with hepatocytotropism.
This case shows cytotoxic immunophenotype of HS-TCL in a cat, which has not been demonstrated before. Severe hepatocytotropism and haemophagocytosis of the neoplastic cells were likely to be associated with jaundice and anaemia, respectively, and the poor outcome of the present case.
一只14岁3个月大的已绝育雌性混血猫出现黄疸、贫血和血小板减少。脾脏细胞学检查后怀疑为与淋巴瘤相关的噬血细胞综合征。尽管使用泼尼松龙、L-天冬酰胺酶和尼莫司汀进行了治疗，但这只猫在初次就诊后176天死亡。尸检显示脾肿大和肝肿大，无淋巴结病。组织病理学检查显示，肿瘤性淋巴细胞浸润肝血窦和脾血窦。肿瘤性淋巴细胞表现出明显的亲肝细胞性，并含有红细胞，电镜检查也证实了这一点。免疫组化检查显示，肿瘤性淋巴细胞CD3、TIA1（GMP-17）和颗粒酶B呈阳性，而CD8、CD20、CD56、CD57、CD79a和Iba1呈阴性。基于这些发现，这只猫被诊断为具有亲肝细胞性的肝脾T细胞淋巴瘤（HS-TCL）。
本病例显示了猫HS-TCL的细胞毒性免疫表型，此前尚未见报道。肿瘤细胞严重的亲肝细胞性和噬血细胞作用可能分别与黄疸和贫血以及本病例的不良预后有关。
