Yoshino Yuka, Chambers James K, Nakamori Taichi, Goto-Koshino Yuko, Nishigaki Kazuo, Tsujimoto Hajime, Matsuki Naoaki, Nakayama Hiroyuki, Uchida Kazuyuki
Laboratory of Veterinary Pathology (Yoshino, Chambers, Nakamori, Nakayama, Uchida).
Molecular Diagnostic Laboratory, Veterinary Medical Center (Goto-Koshino).
J Vet Diagn Invest. 2017 Sep;29(5):707-710. doi: 10.1177/1040638717704239. Epub 2017 Apr 6.
A 4-y-old cat exhibited neurologic signs such as wobbling, right head tilt, and intention tremor, and MRI revealed a mass in the cerebellum. The cat died 5 mo after initial presentation, and no neoplastic lesions, other than the cerebellar mass, were observed at autopsy. Histologically, large atypical cells resembling Hodgkin cells, with single large inclusion-like nucleoli, and those resembling Reed-Sternberg cells, with symmetrically arranged nuclei, had infiltrated the left side of the cerebellum and were admixed with small lymphocytes. These atypical cells were positive for feline leukemia virus (FeLV), CD20, BLA36, vimentin, p16, p53, and Pax5, and negative for CD3, CD79a, and Iba1 by immunohistochemistry. Multiplex PCR for immunoglobulin heavy-chain gene rearrangement revealed monoclonal proliferation of B-lymphocytes. We describe this feline primary cerebellar B-cell lymphoma that displayed Hodgkin lymphoma-like tumor cells with FeLV protein expression.
一只4岁的猫出现了诸如摇晃、右侧头部倾斜和意向性震颤等神经症状，MRI显示小脑有一个肿块。这只猫在初次就诊后5个月死亡，尸检时除了小脑肿块外未观察到其他肿瘤性病变。组织学上，类似霍奇金细胞的大的非典型细胞，具有单个大的包涵体样核仁，以及类似里德-施特恩贝格细胞的细胞，其核对称排列，已浸润到小脑左侧，并与小淋巴细胞混合。通过免疫组织化学，这些非典型细胞对猫白血病病毒（FeLV）、CD20、BLA36、波形蛋白、p16、p53和Pax5呈阳性，而对CD3、CD79a和Iba1呈阴性。免疫球蛋白重链基因重排的多重PCR显示B淋巴细胞的单克隆增殖。我们描述了这种表现出具有FeLV蛋白表达的霍奇金淋巴瘤样肿瘤细胞的猫原发性小脑B细胞淋巴瘤。
