a Department of Kidney, School of Clinical Medicine, Nanjing University of Traditional Chinese Medicine, Nanjing, China.
b Department of Kidney, Nanjing boda kidney hospital affiliated to Nan Jing University Chinese Medicine, Nanjing, China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):2972-2979. doi: 10.1080/21691401.2019.1640712.
Ginsenoside Rg1 exerts a beneficial effect in many kidney diseases. But little work has been done to confirm whether ginsenoside Rg1 could also exert a protective effect on anti-glomerular basement membrane (anti-GBM) glomerular nephritis (GN). We aimed to explore the role of ginsenoside Rg1 in attenuating anti-GBM GN in vitro and in vivo and investigate the mechanism under its action. Interleukin-1β (IL-1β) treated podocytes were used as a cell model. A total of 20 mice were used to build the Anti-GBM GN mice model. Real-time PCR analysis (RT-PCR), Western blot analysis and ELISA assay were conducted to detect related indicators in this study. The statistical analysis was performed using GraphPad Prism software 6.0. Ginsenoside Rg1 attenuates IL-1β-induced inflammation and apoptosis in podocytes. NRF2 expression can be inhibited by IL-1β, whereas reversed by ginsenoside Rg1. NRF2 inhibitor ML385 can significantly reverse the effects of ginsenoside Rg1 on inflammation and apoptosis induced by IL-1β, and block the inhibitory effect of ginsenoside Rg1 on IL-1β activated MAPK pathway. In addition, ginsenoside Rg1 could improve anti-GBM GN injury . Ginsenoside Rg1 inhibits the anti-GBM GN damage through regulating NRF2 pathway and . Our findings provide new insight and mechanism of ginsenoside Rg1 to prevent anti-GBM GN.
人参皂苷 Rg1 在许多肾脏疾病中发挥有益作用。但是，很少有工作证实人参皂苷 Rg1 是否也对抗肾小球基底膜（anti-GBM）肾小球肾炎（GN）具有保护作用。我们旨在探讨人参皂苷 Rg1 在体外和体内减轻抗-GBM GN 的作用及其作用机制。用白细胞介素-1β（IL-1β）处理的足细胞作为细胞模型。总共使用 20 只小鼠来建立抗-GBM GN 小鼠模型。本研究采用实时 PCR 分析（RT-PCR）、Western blot 分析和 ELISA 检测相关指标。统计分析采用 GraphPad Prism 软件 6.0 进行。人参皂苷 Rg1 可减轻 IL-1β诱导的足细胞炎症和凋亡。NRF2 的表达可被 IL-1β抑制，而被人参皂苷 Rg1 逆转。NRF2 抑制剂 ML385 可显著逆转 IL-1β 诱导的人参皂苷 Rg1 对炎症和凋亡的作用，并阻断人参皂苷 Rg1 对 IL-1β激活的 MAPK 通路的抑制作用。此外，人参皂苷 Rg1 可以改善抗-GBM GN 损伤。人参皂苷 Rg1 通过调节 NRF2 通路抑制抗-GBM GN 损伤。我们的研究结果为预防抗-GBM GN 提供了人参皂苷 Rg1 的新见解和机制。
