Wang Ziling, Xia Jieyu, Li Jing, Chen Linbo, Chen Xiongbin, Zhang Yanyan, Wang Lu, Wang Yaping
Laboratory of Stem Cells and Tissue Engineering, Basic Medical College, Chongqing Medical University, Chongqing 400016, China.
Chongqing University Cancer Hospital and Chongqing Cancer Institute and Chongqing Cancer Hospital, Chongqing 400030, China.
Evid Based Complement Alternat Med. 2022 Mar 27;2022:2875583. doi: 10.1155/2022/2875583. eCollection 2022.
Ginsenoside Rg1 is a major component of ginseng with antioxidative and antiaging effects, which is a traditional Chinese medicine. In this study, we investigated the potential spillover and mechanism of action of Rg1 on LiCl-driven hematopoietic stem cell aging.
Collect the purified Sca-1 hematopoietic cells for differentiation ability detection and biochemical and molecular labeling. The experiment found that Rg1 plays an antiaging role in reversing the SA--gal staining associated with LiCl-induced hematopoietic stem cell senescence, the increase in p53 and p21 proteins, and sustained DNA damage. At the same time, Rg1 protects hematopoietic cells from the reduced differentiation ability caused by LiCl. In addition, Rg1 increased the excessive inhibition of intracellular GSK-3 protein, resulting in the maintenance of -catenin protein levels in hematopoietic cells after LiCl treatment. Then, the target gene level of -catenin can be maintained.
Rg1 exerts the pharmacological effect of maintaining the activity of GSK-3 in Sca-1 hematopoietic cells, enhances the antioxidant potential of cells, improves the redox homeostasis, and thus protects cells from the decline in differentiation ability caused by aging. This study provides a potential therapeutic strategy to reduce stem cell pool failure caused by chronic oxidative damage to hematopoietic stem cells.
人参皂苷Rg1是人参的主要成分，具有抗氧化和抗衰老作用，人参是一种传统中药。在本研究中，我们研究了Rg1对氯化锂驱动的造血干细胞衰老的潜在溢出效应及作用机制。
收集纯化的Sca-1造血细胞进行分化能力检测以及生化和分子标记。实验发现，Rg1在逆转与氯化锂诱导的造血干细胞衰老相关的SA-β-半乳糖苷酶染色、p53和p21蛋白增加以及持续性DNA损伤方面发挥抗衰老作用。同时，Rg1保护造血细胞免受氯化锂导致的分化能力降低的影响。此外，Rg1增加了细胞内GSK-3蛋白的过度抑制，导致氯化锂处理后造血细胞中β-连环蛋白水平得以维持。进而，β-连环蛋白的靶基因水平能够得以维持。
Rg1发挥维持Sca-1造血细胞中GSK-3活性的药理作用，增强细胞的抗氧化潜能，改善氧化还原稳态，从而保护细胞免受衰老导致的分化能力下降的影响。本研究提供了一种潜在的治疗策略，以减少造血干细胞慢性氧化损伤导致的干细胞池衰竭。
