Department of Food Phytochemistry, Instituto de la Grasa (Spanish National Research Council, CSIC), Carretera de Utrera km 1, Campus Universidad Pablo de Olavide, edificio 46, 41013 Seville, Spain.
Food Funct. 2019 Aug 1;10(8):4844-4853. doi: 10.1039/c9fo00917e. Epub 2019 Jul 19.
Three olive modified pectin extracts have been produced by heat and acid treatment of the major by-product of olive oil production. Their effect on proliferation of the colon carcinoma Caco-2 and the leukemia monocytic THP-1 cell lines has been studied in order to determine possible anti-tumor properties. All extracts inhibited proliferation at some of the concentrations ranging from 1 to 10 mg ml. Interestingly none of the extracts inhibited the growth of confluent Caco-2 cells, showing the specificity of the antiproliferative effect for the transformed Caco-2 phenotype. All the extracts inhibited agglutination of red blood cells by galectin-3, a lectin involved in tumor growth, metastasis, and immune cell regulation that has been proposed as a mediator of the anti-tumor effects of modified pectins. In addition, activation of caspase-3 in THP-1 cells indicates that treatment with the pectin-rich extracts triggers apoptosis. These results point to a possible use as health-promoting food ingredients or supplements.
三种橄榄改性果胶提取物是通过加热和酸处理橄榄油生产的主要副产物得到的。为了确定其可能的抗肿瘤特性，研究了它们对结肠癌细胞 Caco-2 和白血病单核细胞 THP-1 细胞系增殖的影响。所有提取物在 1 至 10mg/ml 的浓度范围内都抑制了增殖。有趣的是，没有一种提取物能抑制 Caco-2 细胞的汇合生长，这表明抗增殖作用对转化的 Caco-2 表型具有特异性。所有提取物都抑制了半乳糖凝集素-3 对红细胞的凝集，半乳糖凝集素-3 是一种参与肿瘤生长、转移和免疫细胞调节的凝集素，它被认为是改性果胶抗肿瘤作用的介质。此外，THP-1 细胞中 caspase-3 的激活表明，富含果胶的提取物的处理触发了细胞凋亡。这些结果表明，它们可能作为促进健康的食品成分或补充剂使用。
