Department of Geriatrics, Anhui Provincial Hospital, Hefei, Anhui 230000, P.R. China.
Department of Cardiology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200433, P.R. China.
Mol Med Rep. 2019 Sep;20(3):2916-2921. doi: 10.3892/mmr.2019.10484. Epub 2019 Jul 9.
Microparticles are carriers of signals for intracellular signal transduction. These carriers include proteins, mRNAs, microRNAs and other bioactive substances. Platelets are a major source of circulating microparticles, and microparticles are closely associated with the development of certain cardiovascular diseases. In the present study, a method for separating, extracting and identifying platelet‑derived microparticles was developed and differences in the expression of surface proteins on microparticles harvested from platelets stimulated by vortexing or treatment with thrombin was investigated. The counts, composition, sizes and inner structures of microparticles were determined using flow cytometry and transmission electron microscopy. Additionally, it was demonstrated that platelets could be readily activated, and a large quantity of microparticles with varying complex compositions, structures and sizes were derived from activated platelets. High purity platelet‑derived microparticles were obtained by gradient centrifugation. However, the microparticles derived from platelets stimulated by thrombin treatment or vortexing differed significantly in the levels of CD63. The present study aimed to provide improved options for the extraction and identification of microparticles.
微粒体是细胞内信号转导的信号载体。这些载体包括蛋白质、mRNA、microRNA 和其他生物活性物质。血小板是循环微粒体的主要来源，微粒体与某些心血管疾病的发展密切相关。本研究开发了一种分离、提取和鉴定血小板衍生微粒体的方法，并研究了涡旋或凝血酶处理刺激的血小板中提取的微粒体表面蛋白表达的差异。使用流式细胞术和透射电子显微镜确定了微粒体的计数、组成、大小和内部结构。此外，还证明了血小板很容易被激活，并且可以从激活的血小板中获得具有不同复杂组成、结构和大小的大量微粒体。通过梯度离心获得高纯度的血小板衍生微粒体。然而，经凝血酶处理或涡旋刺激的血小板衍生的微粒体在 CD63 水平上有显著差异。本研究旨在为微粒体的提取和鉴定提供更好的选择。
