Department of Medicine, Changde Vocational Technical College, Changde, Hunan 415000, P.R. China.
Mol Med Rep. 2019 Sep;20(3):2533-2540. doi: 10.3892/mmr.2019.10486. Epub 2019 Jul 11.
The aim of the present study was to investigate the effect of microRNA (miR)‑539 on the proliferation and migration of gastric cancer cells, and explore the underlying mechanism. Gastric cancer cell lines with high or low miR‑539 and SRY‑box 5 (SOX5) expression levels were constructed by transfection. The proliferation of gastric cancer cells was then detected by Cell Counting Kit‑8 assay and cell migration was tested by transwell assay. The results revealed low expression of miR‑539 and high expression of SOX5 in gastric cancer tissues and cells as compared with the levels in normal tissues and cells, suggesting that there was a negative correlation between miR‑539 and SOX5. Dual‑luciferase reporter experiments demonstrated that miR‑539 directly targeted SOX5. The proliferation and migration of gastric cancer cells were negatively regulated by the overexpression of miR‑539, while positively regulated by the overexpression of SOX5. Notably, SOX5 overexpression attenuated the inhibitory effect of miR‑539 on gastric cancer cells. The results suggested that SOX5 is a target gene of miR‑539, and that miR‑539 inhibits the proliferation and migration of gastric cancer cells by targeting SOX5.
本研究旨在探讨微小 RNA（miR）-539 对胃癌细胞增殖和迁移的影响，并探讨其潜在机制。通过转染构建 miR-539 表达水平高或低和性别决定区 Y 框 5（SOX5）表达水平高或低的胃癌细胞系。然后通过细胞计数试剂盒-8 检测法检测胃癌细胞的增殖，通过 Transwell 检测法检测细胞迁移。结果显示，与正常组织和细胞相比，胃癌组织和细胞中 miR-539 表达水平较低，SOX5 表达水平较高，提示 miR-539 与 SOX5 呈负相关。双荧光素酶报告基因实验表明，miR-539 可直接靶向 SOX5。miR-539 的过表达可负向调节胃癌细胞的增殖和迁移，而 SOX5 的过表达则可正向调节。值得注意的是，SOX5 的过表达可减弱 miR-539 对胃癌细胞的抑制作用。结果提示 SOX5 是 miR-539 的靶基因，miR-539 通过靶向 SOX5 抑制胃癌细胞的增殖和迁移。
