Huang Lingting, Yu Zhonghua, Chen Jialun, Tang Dianping
Key Laboratory for Analytical Science of Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, People's Republic of China.
ACS Appl Bio Mater. 2020 Dec 21;3(12):9156-9163. doi: 10.1021/acsabm.0c01447. Epub 2020 Dec 4.
An effective signal amplification strategy based on temperature-amplification synergistic pressure was designed for the sensitive detection of carcinoembryonic antigen (CEA) by using a noncontact point-of-care testing (POCT) aptasensor with a flexible pressure sensor based on a poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS)-modified sponge (PEDOT:PSS-sponge). In the biological recognition system, target analyte triggered the release of platinum nanoparticle-labeled complementary DNA (Pt-cDNA) from CEA aptamer-conjugated magnetic beads. On the basis of the unique characteristics of platinum nanoparticles (e.g., catalytic ability for HO decomposition, peroxidase-like catalytic activity, and photothermal characteristics), the platinum nanoparticles (PtNPs) could not only catalyze hydrogen peroxide (HO) to produce a large amount of oxygen (O) but could also assist the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to a photothermal agent ox-TMB with the increasing temperature. Under 808 nm near-infrared (NIR) laser irradiation, an increase in the pressure and temperature occurred simultaneously in the closed detection cell. Meanwhile, the increasing temperature could be helpful for further increasing the pressure. The flexible pressure sensor was compressed in a closed system to cause a decrease in contact resistance, thereby establishing a correlation between the concentration and the resistance-readable analysis. Under optimum conditions, the dynamic detection range of this detection strategy for target CEA was between 0.2 and 80 ng mL, and the limit of detection (LOD) was 0.15 ng mL. Overall, the signal amplification strategy of temperature coordination provides possibilities for the future development of sensitive and portable POCT protocols.
基于温度放大协同压力设计了一种有效的信号放大策略，用于通过使用基于聚（3,4-乙撑二氧噻吩）：聚（苯乙烯磺酸盐）（PEDOT:PSS）修饰海绵（PEDOT:PSS-海绵）的柔性压力传感器的非接触式即时检测（POCT）适体传感器灵敏检测癌胚抗原（CEA）。在生物识别系统中，目标分析物触发铂纳米颗粒标记的互补DNA（Pt-cDNA）从CEA适体偶联磁珠中释放。基于铂纳米颗粒的独特特性（例如，对HO分解的催化能力、类过氧化物酶催化活性和光热特性），铂纳米颗粒（PtNPs）不仅可以催化过氧化氢（HO）产生大量氧气（O），还可以随着温度升高辅助将3,3',5,5'-四甲基联苯胺（TMB）氧化为光热剂ox-TMB。在808 nm近红外（NIR）激光照射下，封闭检测池中压力和温度同时升高。同时，温度升高有助于进一步增加压力。柔性压力传感器在封闭系统中被压缩，导致接触电阻降低，从而建立浓度与电阻可读分析之间的相关性。在最佳条件下，该检测策略对目标CEA的动态检测范围为0.2至80 ng mL，检测限（LOD）为0.15 ng mL。总体而言，温度协同信号放大策略为灵敏且便携的POCT方案的未来发展提供了可能性。
