1 Agriculture, Food and Resource Sciences, University of Maryland, Eastern Shore, Princess Anne, Maryland 21853.
2 Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida 32611, USA.
J Food Prot. 2019 Aug;82(8):1364-1368. doi: 10.4315/0362-028X.JFP-18-552.
serovar Typhimurium is one of the leading causes of nontyphoidal gastroenteritis of humans in the United States. Commercially processed poultry carcasses are frequently contaminated with serovar Kentucky in the United States. The aim of the study was to detect the virulence plasmid containing the genes from isolates recovered from commercially processed chicken carcasses. A total of 144 isolates ( Typhimurium, = 72 and Kentucky, = 72) were used for isolation of plasmids and detection of corresponding virulence genes ( and ). Only four (5.5%) Typhimurium isolates tested positive for all three virulence genes and hence were classified as possessing the virulence plasmid. All isolates of Kentucky were negative for the virulence plasmid and genes. These results indicate that the virulence plasmid, which is very common among clinical isolates of Typhimurium and other serovars (e.g., Enteritidis, Dublin, Choleraesuis, Gallinarum, Pullorum, and Abortusovis), may not be present in a significant portion of commercially processed chicken carcass isolates.
鼠伤寒血清型是美国引起非伤寒性胃肠炎的主要原因之一。在美国，商业加工的禽肉胴体经常被肯塔基血清型污染。本研究的目的是从商业加工的鸡胴体中分离出携带 基因的毒力质粒。共分离出 144 株（鼠伤寒血清型，=72 株，肯塔基血清型，=72 株），用于分离质粒和检测相应的毒力基因（和）。只有 4 株（5.5%）鼠伤寒血清型分离株对所有 3 种毒力基因均呈阳性反应，因此被归类为携带毒力质粒。所有的 肯塔基血清型分离株均未携带毒力质粒和基因。这些结果表明，毒力质粒在鼠伤寒血清型和其他血清型（如肠炎血清型、都柏林血清型、霍乱血清型、鸡血清型、鸡白痢血清型和流产血清型）的临床分离株中非常常见，但在商业加工的鸡胴体分离株中可能并不存在于很大一部分中。
