School of Nursing, Qingdao University, Qingdao, China.
Department of Urology, Qingdao Municipal Hospital, Qingdao University, Qingdao, China.
Neurogastroenterol Motil. 2019 Oct;31(10):e13677. doi: 10.1111/nmo.13677. Epub 2019 Jul 19.
This study was to inspect the antidepressant-like effect of prebiotics and probiotics, and to explore the effect of modulating gut microbiota on the serotonin (5-HT) metabolism.
Fifty rats were separated into control and other four groups randomly. The four groups underwent the chronic unpredictable mild stress (CUMS) intervention with or without prebiotics and probiotics (Bifidobacterium longum, L. rhamnosus) treatment. After weighted, the animals underwent a series of behavioral tests comprising the sucrose preference test (SPT) and the forced swimming test (FST). Central and colonic serotonin levels and relative metabolism factors were measured and analyzed. Microbiota was examined by 16S rRNA gene pyrosequencing.
CUMS intervention caused a decrease in body weight, an increase in FST, and a decrease in SPT. Prebiotics and probiotics all ameliorated the CUMS-induced loss of weight and depressive-like behaviors to a certain extent, especially L. rhamnosus. Compared with the group of CUMS intervention, the rats of probiotics and probiotics treatment had a tendency to reduce colonic 5-HT and increase 5-HT in frontal cortex and hippocampus. However, there was no significant difference in peripheral blood 5-HT among these groups. Furthermore, CUMS caused noteworthy gut microbiota variations at the phylum and other levels in rats. Remarkably, there were considerable relations of perturbed gut microbiota with the changed metabolism of 5-HT.
In conclusion, these findings implied that prebiotics and probiotics have antidepressive effects, and a considerable effect on the regulation of 5-HT metabolism, especially L. rhamnosus.
本研究旨在观察益生元和益生菌的抗抑郁作用，并探讨调节肠道微生物群对 5-羟色胺（5-HT）代谢的影响。
将 50 只大鼠随机分为对照组和其他四组。四组均采用慢性不可预测轻度应激（CUMS）干预，同时给予或不给予益生元和益生菌（长双歧杆菌、鼠李糖乳杆菌）治疗。称重后，动物进行一系列行为测试，包括蔗糖偏好测试（SPT）和强迫游泳测试（FST）。测量和分析中枢和结肠 5-HT 水平及相关代谢因子。采用 16S rRNA 基因焦磷酸测序法检测微生物群。
CUMS 干预导致体重下降、FST 增加和 SPT 减少。益生元和益生菌都在一定程度上改善了 CUMS 引起的体重减轻和抑郁样行为，尤其是鼠李糖乳杆菌。与 CUMS 干预组相比，益生菌和益生菌处理组大鼠结肠 5-HT 降低，额皮质和海马 5-HT 升高的趋势，但各组间外周血 5-HT 无明显差异。此外，CUMS 导致大鼠肠道微生物群在门和其他水平发生显著变化。值得注意的是，肠道微生物群的紊乱与 5-HT 代谢的改变有很大关系。
综上所述，这些发现表明益生元和益生菌具有抗抑郁作用，并对 5-HT 代谢的调节有显著作用，尤其是鼠李糖乳杆菌。
