北方蛇头鱼（Channa argus）髓过氧化物酶在维氏气单胞菌感染中的作用。
Role of Myeloperoxidase of northern snakehead (Channa argus) in Aeromonas veronii infection.
机构信息
College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, Jilin, 130118, China.
College of Animal Science and Technology, Northwest A&F University, Yangling, Shanxi, 712100, PR China.
出版信息
Microb Pathog. 2019 Oct;135:103622. doi: 10.1016/j.micpath.2019.103622. Epub 2019 Jul 17.
Myeloperoxidase (MPO) is a ferrous lysosomal protein with many immune functions that belongs to the heme peroxidase enzyme. In this study, the functions of MPO in the northern snakehead (Channa argus) were investigated by cloning an MPO cDNA sequence with a full length of 3181 bp. Homology analysis showed that northern snakehead MPO gene had the highest (81%) homology with mandarin fish (Siniperca chuatsi). In healthy northern snakehead, the MPO gene was expressed in the head-kidney, kidney, heart, gill, spleen, liver, and muscles but not midgut. After the northern snakehead was infected with Aeromonas veronii, the MPO gene expression varied in different tissues with low level in spleen, liver, gill and muscle, fluctuated in kidney and head-kidney and showed high level in heart. The result indicated that MPO might play an important role in the antimicrobial immune response of the northern snakehead.
髓过氧化物酶（MPO）是一种具有多种免疫功能的亚铁溶酶体蛋白，属于血红素过氧化物酶酶。本研究通过克隆全长为 3181bp 的 MPO cDNA 序列，探讨了 MPO 在北方蛇头（Channa argus）中的功能。同源性分析表明，北方蛇头 MPO 基因与鳜鱼（Siniperca chuatsi）的同源性最高（81%）。在健康的北方蛇头中，MPO 基因在头部-肾脏、肾脏、心脏、鳃、脾脏、肝脏和肌肉中表达，但不在中肠中表达。北方蛇头感染维氏气单胞菌后，MPO 基因在不同组织中的表达水平不同，脾脏、肝脏、鳃和肌肉中表达水平较低，肾脏和头部-肾脏中波动，心脏中表达水平较高。结果表明，MPO 可能在北方蛇头的抗菌免疫反应中发挥重要作用。
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