压力变化对超临界流体萃取野生胡萝卜（Daucus carota subsp. maritimus）提取物效率的影响。

Effect of pressure variation on the efficiency of supercritical fluid extraction of wild carrot (Daucus carota subsp. maritimus) extracts.

机构信息

Research Unit 13ES63, Applied Chemistry and Environment, Monastir University, Faculty of Sciences of Monastir, Monastir 5000, University of Monastir, Tunisia.

Department of Life Sciences, Laboratory of Botany and Plant Ecology, Faculty of Sciences of Bizerta, Jarzouna-7021, Bizerta, University of Carthage, Tunisia; Department Pharmaceutical Sciences "A", Laboratory of Botany, Cryptogamy and Plant Biology, Faculty of Pharmacy of Monastir, BP 207, Avenue Avicenna-5000, University of Monastir, Tunisia; Department of Silvo-Pastoral Resources, Laboratory of Silvo-Pastoral Resources, Silvo-Pastoral Institute of Tabarka, BP. 345, Tabarka 8110, University of Jendouba, Tunisia.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Sep 1;1125:121713. doi: 10.1016/j.jchromb.2019.121713. Epub 2019 Jul 10.

DOI:10.1016/j.jchromb.2019.121713

PMID:31323559

Abstract

The present study was conducted to check the comparative qualities of essential oils prepared by hydrodistillation (HD) and supercritical fluid techniques. It constitutes the first attempt to investigate the chemical composition of Daucus carota subsp. maritimus extracts using supercritical fluid technology (SFE) as an environmentally clean innovative method of extraction. The effect of pressure on the nature of extractable substances from wild carrot has been performed at a constant temperature of 50 °C and two different pressures (100 and 300 bar). The experimental results showed that pressure had a significant enhancing effect on the fluid transport properties and therefore on yield values. The extraction yield increases from 1.167 to 2.986% while increasing pressure. The chemical compositions of the essential oils prepared by HD and SFE were analyzed on the basis of gas chromatography-mass spectroscopy (GC-MS). Thus, we noticed that all analyzed samples were enriched in geranyl acetate and β‑bisabolene, and that the quantities of different identified substances were extremely sensitive to the extraction method and to the pressure variation in case of SFE.

摘要

本研究旨在检查水蒸气蒸馏（HD）和超临界流体技术制备的精油的比较质量。这是首次尝试使用超临界流体技术（SFE）作为一种环保的创新提取方法来研究海洋野胡萝卜的化学成分。在 50°C 的恒定温度和两个不同压力（100 和 300 巴）下，研究了压力对野生胡萝卜可提取物性质的影响。实验结果表明，压力对流体输送性质有显著的增强作用，因此对产率值有影响。萃取产率从 1.167%增加到 2.986%，同时压力增加。根据气相色谱-质谱联用（GC-MS）分析了 HD 和 SFE 制备的精油的化学成分。因此，我们注意到所有分析的样品都富含乙酸香叶酯和β- 大根香叶烯，并且不同鉴定物质的数量对提取方法和 SFE 中的压力变化极为敏感。