Department of Molecular Biology and Genetics, Democritus University of Thrace, University Campus-Dragana, 68100 Alexandroupolis, Greece.
VIORYL S.A., Chemical & Agricultural Industry, Research S.A., 19014 Afidnes, Greece.
Molecules. 2019 Jul 18;24(14):2612. doi: 10.3390/molecules24142612.
species are plants rich in volatile oils that are mainly used for culinary purposes. In recent years, there has been a growing interest in the biological activities of their essential oils. L. is a plant mainly found in Greece, Turkey, and Sicily, whose oil is rich in carvacrol, a highly bioactive phytochemical. The aim of this study was to analyze the chemical composition of essential oil (OOEO), and investigate its potential anticancer effects in vitro and in vivo. GC/MS analysis identified carvacrol as OOEO's main constituent. In vitro antiproliferative activity was assayed with the sulforhodamine B (SRB) assay against human cancer cell lines from four tumor types. HT-29, a colorectal cancer cell line, was the most sensitive to the antiproliferative activity of OOEO. Wound-healing assay and Annexin V-PI staining were employed to investigate the antimigratory and the pro-apoptotic potential of OOEO, respectively, against human (HT-29) and murine (CT26) colon cancer cells. Notably, OOEO attenuated migration and induced apoptosis-related morphological changes in both cell lines. Prophylactic oral administration of the oil in a BALB/c experimental mouse model inhibited the growth of syngeneic CT26 colon tumors. As far as we know, this is the first report on the antitumor potential of orally administered OOEO.
该物种是富含挥发油的植物，主要用于烹饪用途。近年来，人们对其精油的生物活性越来越感兴趣。L. 是一种主要存在于希腊、土耳其和西西里岛的植物，其油富含具有高度生物活性的植物化学物质香芹酚。本研究旨在分析精油（OOEO）的化学成分，并研究其在体外和体内的潜在抗癌作用。GC/MS 分析鉴定香芹酚为 OOEO 的主要成分。用磺基罗丹明 B（SRB）法测定了对来自四种肿瘤类型的人类癌细胞系的体外增殖活性。HT-29，一种结直肠癌细胞系，对 OOEO 的增殖活性最敏感。划痕愈合试验和 Annexin V-PI 染色分别用于研究 OOEO 对人（HT-29）和鼠（CT26）结肠癌细胞的抗迁移和促凋亡潜力。值得注意的是，OOEO 减弱了两种细胞系的迁移并诱导了与凋亡相关的形态变化。在 BALB/c 实验小鼠模型中预防性口服给予该油抑制了同源 CT26 结肠肿瘤的生长。据我们所知，这是口服给予 OOEO 的抗肿瘤潜力的首次报道。
