Laboratory of Research on Biological Systems and Geomatics (L.R.S.B.G), Department of Agronomy, Faculty of Life and Natural Sciences, University Mustapha Stambouli of Mascara, Mascara, Algeria.
Folia Microbiol (Praha). 2024 Aug;69(4):835-846. doi: 10.1007/s12223-023-01126-z. Epub 2024 Jan 4.
Three endophytic bacteria, namely BvV, BvP and BvL, were newly isolated from the root nodules of bean, pea and lentil plants respectively cultivated in Mascara the northwest of Algeria, and identified by 16S ribosomal RNA gene sequencing as Brevundimonas naejangsanensis. These strains were able to produce hydrolytic enzymes and hydrogen cyanide. All strains produced a growth-promoting hormone, indole acetic acid, varying in concentration from 83.2 to 171.7 µg/mL. The phosphate solubilizing activity of BvV, BvP and BvL varied from 25.5 to 42.02 µg/mL for tricalcium phosphate. The three antagonistic Brevundimonas spp. showed in vitro the most inhibitory effect on mycelial growth of Fusarium redolens FRC (from 78.33 to 85.55%). Strain BvV, BvP and BvL produced also volatile metabolites which inhibited mycelial FRC growth up to 39.2%. All strains showed significant disease reduction in pot experiments. Chickpea Fusarium yellows severity caused by FRC was reduced significantly from 89.3 to 96.6% in the susceptible cultivar ILC 482 treated with antagonistic B. naejangsanensis. The maximum stimulatory effect on chickpea plants growth was observed by inoculation of strain BvV. This treatment resulted in a 7.40-26.21% increase in shoot height as compared to the control plants. It is concluded that the endophytic bacterial strains of B. naejangsanensis having different plant growth promoting (PGP) activities can be considered as beneficial microbes for sustainable agriculture. To our knowledge, this is the first report to use B. naejangsanensis strains as a new biocontrol agent against F. redolens, a new pathogen of chickpea plants causing Fusarium yellows disease in Algeria.
从分别在阿尔及利亚西北部马萨拉种植的豆类、豌豆和小扁豆植物的根瘤中分离出新的内生细菌 BvV、BvP 和 BvL，通过 16S 核糖体 RNA 基因测序鉴定为鞘氨醇单胞菌。这些菌株能够产生水解酶和氢氰酸。所有菌株都产生了生长促进激素吲哚乙酸，浓度从 83.2 到 171.7 µg/mL 不等。BvV、BvP 和 BvL 对磷酸三钙的解磷活性从 25.5 到 42.02 µg/mL 不等。三种拮抗鞘氨醇单胞菌在体外对红镰孢菌 FRC 的菌丝生长表现出最强的抑制作用（从 78.33 到 85.55%）。菌株 BvV、BvP 和 BvL 还产生了挥发性代谢物，抑制 FRC 菌丝生长高达 39.2%。所有菌株在盆栽试验中均表现出显著的防病效果。在易感品种 ILC 482 中，接种拮抗鞘氨醇单胞菌可显著降低由 FRC 引起的鹰嘴豆黄萎病的严重程度，从 89.3%降至 96.6%。接种菌株 BvV 对鹰嘴豆植株生长的最大刺激作用。与对照植株相比，该处理使茎高增加了 7.40-26.21%。结论是，具有不同植物促生（PGP）活性的鞘氨醇单胞菌内生菌株可被视为可持续农业的有益微生物。据我们所知，这是首次使用鞘氨醇单胞菌菌株作为一种新的生防剂来防治红镰孢菌的报道，红镰孢菌是一种新的豌豆病原菌，在阿尔及利亚引起黄萎病。
