Yuasa I, Umetsu K, Suenaga K, Ito K, Iha M, Hirata H, Robinet-Lévy M, Inoue T, Okada K
Department of Legal Medicine, Tottori University School of Medicine, Yonago, Japan.
Hum Hered. 1988;38(2):91-4. doi: 10.1159/000153764.
Factor I (C3b inactivator) polymorphism in the Japanese (in Western and Southern Japan), Taiwanese, Nepalese and French was studied using isoelectric focusing on polyacrylamide gels. The exposure of passively blotted nitrocellulose membranes to glutaraldehyde vapor facilitated the subsequent immunodetection of a low concentration of factor I and permitted the reliable identification of the three phenotypes determined by two codominant alleles FIA and FIB. The data indicated a west-to-east genocline, ranging from France to Western Japan, in which FI*A changed from 0.006 to 0.120.
利用聚丙烯酰胺凝胶等电聚焦技术，对日本（日本西部和南部）、台湾地区、尼泊尔和法国人群中的Ⅰ因子（C3b灭活因子）多态性进行了研究。将被动印迹的硝酸纤维素膜暴露于戊二醛蒸汽中，有助于随后对低浓度Ⅰ因子进行免疫检测，并能可靠地鉴定由两个共显性等位基因FIA和FIB所决定的三种表型。数据表明，存在一条从法国到日本西部的由西向东的基因渐变群，其中FI*A的频率从0.006变化到0.120。
