通过等电聚焦和免疫印迹法测定α2HS-糖蛋白表型：冲绳地区HSGA*5的多态性出现情况

Determination of alpha 2HS-glycoprotein phenotypes by isoelectric focusing and immunoblotting: polymorphic occurrence of HSGA*5 in Okinawa.

作者信息

Yuasa I, Taira T, Suenaga K, Ito K, Okada K

出版信息

Hum Genet. 1985;70(1):32-4. doi: 10.1007/BF00389454.

Abstract

A print immunofixation is a very useful procedure for the demonstration of alpha 2HS-glycoprotein (HSGA) following polyacrylamide gel isoelectric focusing. However, this technique has the one disadvantage of requiring a large volume of expensive antiserum. In this paper an alternative detection system is presented which involves the non-electric transfer of HSGA from a focused gel to a nitrocellulose filter and the immunologic detection of HSGA immobilized on nitrocellulose. Using this method the distribution of HSGA polymorphism in the Nepalese and Japanese populations was investigated. All the populations tested were found to lack the common HSGA3 of the Caucasians. A rare HSGA5 in Honshu, a main island of Japan, was observed at polymorphic frequency in Okinawa, Southern Japan.

摘要

蛋白质印迹免疫固定法是在聚丙烯酰胺凝胶等电聚焦后用于显示α2HS-糖蛋白（HSGA）的一种非常有用的方法。然而，该技术有一个缺点，即需要大量昂贵的抗血清。本文介绍了一种替代检测系统，该系统涉及将HSGA从聚焦凝胶非电转移到硝酸纤维素滤膜上，并对固定在硝酸纤维素上的HSGA进行免疫检测。使用这种方法，研究了尼泊尔人和日本人中HSGA多态性的分布。所有测试人群均未发现高加索人常见的HSGA3。在日本主岛本州罕见的HSGA5，在日本南部冲绳以多态频率被观察到。