Moosdeen F, Williams J D, Secker A
Department of Medical Microbiology, London Hospital Medical College, UK.
J Antimicrob Chemother. 1988 Apr;21(4):439-43. doi: 10.1093/jac/21.4.439.
Many methods of disc susceptibility testing aim at an inoculum to yield a semi-confluent growth. We have improved the means of standardizing inoculum by measuring the turbidity with a spectrophotometer. Absorbance measurements were made for various bacterial suspensions and dilutions thereof, from 1 in 2 to 1 in 1000. Volumes of 0.1 ml of each dilution were spread on to Diagnostic Sensitivity Test agar. Viable counts of bacteria were made for each absorbance reading and for what constituted semi-confluent growth. Suspensions of bacteria having similar absorbance readings contained variable numbers of viable bacteria depending on the species. The number of bacterial cells that yielded semi-confluent growth also varied with different bacterial species. A chart was prepared to indicate the appropriate dilutions of bacterial suspensions with different absorbance readings to produce semi-confluent growth in sensitivity testing of each species.
许多椎间盘药敏试验方法旨在获得能产生半融合生长的接种物。我们通过用分光光度计测量浊度改进了接种物标准化方法。对各种细菌悬液及其从1:2到1:1000的稀释液进行了吸光度测量。将每种稀释液0.1毫升的体积铺在诊断敏感性试验琼脂上。对每个吸光度读数以及构成半融合生长的情况进行了细菌活菌计数。具有相似吸光度读数的细菌悬液所含活菌数量因菌种而异。产生半融合生长的细菌细胞数量也因不同细菌种类而有所不同。绘制了一张图表，以表明在每种菌种的敏感性试验中，具有不同吸光度读数的细菌悬液的适当稀释度，以产生半融合生长。
