Shi Xu, Wang Wei, Zheng Shufang, Zhang Qiaojian, Xu Shiwen
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Biol Trace Elem Res. 2020 Apr;194(2):525-535. doi: 10.1007/s12011-019-01789-1. Epub 2019 Jul 19.
Selenomethionine is able to relieve the effect of inflammation in various tissues and organs. However, there are few studies about the influences of organic selenium resisting inflammation induced by LPS in chicken trachea. Therefore, the purpose of this experiment is to explore the organic selenium (selenomethionine) can raise immune function and relieve the LPS-induced inflammation of chicken trachea via inhibiting the NF-κB pathway. To investigate the mechanism of organic selenium on chicken trachea, the supplement of selenomethionine and/or LPS-induced chicken models were established. One hundred 46-week-old isa chickens were randomly divided into four groups (n = 25). The four groups were the control group, the selenomethionine group (Se group), the LPS-induced group (LPS group), and the Se and LPS interaction group (Se + LPS group). Then, the expressions of inflammatory factors (including induced nitric oxide synthase (iNOS), nuclear factor-kappa B(NF-κB), tumor necrosis factor (TNF-α), cyclooxygenase-2 (COX-2), and prostaglandin E (PTGEs) synthase), inflammation-related cytokines (including interleukin (IL-2, IL-6, IL-8, IL-17) and immunoglobulin (IgA, IgM, IgY)), the marker of immune function (avian β-defensins (AvBD6, AvBD7)), heat shock proteins (including HSP60, HSP90), and selenoproteins (including Selo, Sels, Selm, Selh, Selu, Seli, SPS2, GPx1, GPx2, Dio1, Sepx1, Sep15, Sepp1, Txnrd1) were detected in our experiment. The above genes were significantly changed in different groups (p < 0.05). We can conclude that organic selenium can increase the function of immunity and the expression of selenoproteins, and mitigate the inflammation induced by LPS via suppression of the NF-κB pathway.
硒代蛋氨酸能够减轻各种组织和器官中的炎症效应。然而，关于有机硒抵抗脂多糖（LPS）诱导的鸡气管炎症影响的研究较少。因此，本实验的目的是探究有机硒（硒代蛋氨酸）是否能通过抑制核因子κB（NF-κB）途径提高免疫功能并减轻LPS诱导的鸡气管炎症。为了研究有机硒对鸡气管的作用机制，建立了补充硒代蛋氨酸和/或LPS诱导的鸡模型。将100只46周龄的伊莎鸡随机分为四组（n = 25）。四组分别为对照组、硒代蛋氨酸组（Se组）、LPS诱导组（LPS组）和Se与LPS联合组（Se + LPS组）。然后，检测了炎症因子（包括诱导型一氧化氮合酶（iNOS）、核因子κB（NF-κB）、肿瘤坏死因子（TNF-α）、环氧化酶-2（COX-2）和前列腺素E（PTGEs）合酶）、炎症相关细胞因子（包括白细胞介素（IL-2、IL-6、IL-8、IL-17）和免疫球蛋白（IgA、IgM、IgY））、免疫功能标志物（禽β-防御素（AvBD6、AvBD7））、热休克蛋白（包括HSP60、HSP90）和硒蛋白（包括Selo、Sels、Selm、Selh、Selu、Seli、SPS2、GPx1、GPx2、Dio1、Sepx1、Sep15, Sepp1、Txnrd1）在本实验中的表达。上述基因在不同组中发生了显著变化（p < 0.05）。我们可以得出结论，有机硒可以增强免疫功能和硒蛋白的表达，并通过抑制NF-κB途径减轻LPS诱导的炎症。
