School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, Shaanxi Province, The People's Republic of China.
Key Laboratory of Membrane Separation of Shaanxi Province, Xi'an University of Architecture and Technology, Xi'an, Shaanxi Province, The People's Republic of China.
J Appl Microbiol. 2019 Nov;127(5):1442-1453. doi: 10.1111/jam.14390. Epub 2019 Aug 1.
The aim of this study was to elucidate the chemical properties and applications of trehalose lipids produced by Rhodococcus qingshengii strain FF and optimize its production yield.
Strain FF was identified as R. qingshengii. It was observed to produce biosurfactants in the presence of n-hexadecane. The biosurfactants were identified as the mixture of trehalose triesters and trehalose tetraesters, mainly consisting of TrehC C C C :10, TrehC C C :6, TrehC C C :5 and TrehC C C :5 based on the analysis of thin layer chromatography, Fourier transform infrared and flight tandem mass spectrometry. The best carbon source and nitrogen source for producing trehalose lipids was the mixture of n-hexadecane and oleic acid (m : m = 1 : 1) and the organic nitrogen, urea. Under this condition, the production of trehalose lipids could reach 7·97 g l . The crude trehalose lipids showed extremely high surface-active properties and were proven to promote the degradation of naphthalene.
The trehalose lipids produced by R. qingshengii strain FF exhibited high surfactant activity under various conditions and were proven to promote the degradation of naphthalene.
Rhodococcus qingshengii strain FF is a potential candidate for bioremediation. The trehalose lipids might be used as unique biosurfactants in cosmetic industries, biological formulations and other applications.
本研究旨在阐明青枯雷尔氏菌 FF 菌株产生的海藻糖脂的化学性质和应用，并优化其产量。
FF 菌株被鉴定为青枯雷尔氏菌。在存在正十六烷的情况下，观察到其产生生物表面活性剂。生物表面活性剂被鉴定为海藻糖三酯和海藻糖四酯的混合物，主要由 TrehC C C C :10、TrehC C C :6、TrehC C C :5 和 TrehC C C :5 组成，基于薄层色谱、傅里叶变换红外和飞行串联质谱分析。产生海藻糖脂的最佳碳源和氮源是正十六烷和油酸（m:m=1:1）和有机氮源，尿素。在这种条件下，海藻糖脂的产量可达 7.97g·l -1 。粗海藻糖脂表现出极高的表面活性，并被证明能促进萘的降解。
青枯雷尔氏菌 FF 菌株产生的海藻糖脂在各种条件下表现出高表面活性剂活性，并被证明能促进萘的降解。
青枯雷尔氏菌 FF 菌株是生物修复的潜在候选菌株。海藻糖脂可能作为独特的生物表面活性剂应用于化妆品工业、生物制剂和其他应用中。
