Laboratory of Applied and Basic Microbiology, Biology and Chemistry Faculty, Universidad de Santiago de Chile, Santiago, Chile; Institute of Biosciences, Chemistry and Physics Faculty, TU Bergakademie Freiberg, 09599, Freiberg, Germany.
Institute of Biosciences, Chemistry and Physics Faculty, TU Bergakademie Freiberg, 09599, Freiberg, Germany.
J Biotechnol. 2018 Aug 20;280:38-41. doi: 10.1016/j.jbiotec.2018.06.001. Epub 2018 Jun 4.
Biosurfactants are amphipathic molecules with relevance in biotechnology due to their structural diversity, low toxicity and biodegradability. The genus Rhodococcus has extensively been studied because of its capacity to produce trehalose-containing surfactants as well as trehalose lipids as potential pathogenic factor. Here we present the draft genome sequence of Rhodococcus erythropolis B7g isolated with toluene from fuel-contaminated soil. The genome comprises 7,175,690 bp in 121 contigs, a G + C content of 62,4% and 7,153 coding DNA sequences (CDSs), and it contains genes for trehalose biosynthesis and surfactant production. Additionally, genes for the production of trehalose-tetraester biosurfactant were identified, whose function was experimentally verified making the strain B7g a potential candidate for use in bioremediation applications or in biosurfactant exploration.
生物表面活性剂是具有两亲性的分子，由于其结构多样性、低毒性和可生物降解性，在生物技术中有重要的应用。红球菌属因其能够产生含有海藻糖的表面活性剂以及海藻糖脂作为潜在的致病因子而被广泛研究。在这里，我们介绍了一株从受污染燃料土壤中用甲苯分离出的红球菌（Rhodococcus erythropolis）B7g 的基因组草图序列。该基因组由 121 个连续序列组成，大小为 7175690bp，G+C 含量为 62.4%，包含 7153 个编码 DNA 序列（CDS），其中有海藻糖生物合成和表面活性剂生产的相关基因。此外，还鉴定了产生海藻糖四酯生物表面活性剂的基因，其功能通过实验得到了验证，这使得菌株 B7g 成为生物修复应用或生物表面活性剂开发的潜在候选菌株。
