维生素D受体基因多态性（rs10735810）与慢性牙周炎的关联
Association of vitamin D receptor gene polymorphism (rs10735810) and chronic periodontitis.
作者信息
Murthykumar Karthikeyan, Arjunkumar Radhika, Jayaseelan Vijayashree Priyadharsini
机构信息
Department of Periodontics, Saveetha Dental College, Chennai, India.
Biomedical Research Unit, Laboratory animal Center, Dental Research Cell, Saveetha Dental College, Chennai, India.
出版信息
J Investig Clin Dent. 2019 Nov;10(4):e12440. doi: 10.1111/jicd.12440. Epub 2019 Jul 19.
AIM
The aim of the present study was to analyze the association between vitamin D receptor (VDR) (rs10735810) gene polymorphism and chronic periodontitis (CP).
METHODS
A total of 100 subjects were recruited for this study, which included 50 CP and 50 healthy controls. Genomic DNA was extracted from the whole blood collected from the subjects. DNA was amplified using specific primers flanking the FokI region of the VDR gene (rs10735810). The amplicon was further subjected to genotyping using restriction fragment length polymorphism (RFLP) using the FokI enzyme. The genotype obtained based on RFLP pattern was recorded and used for statistical analysis. The distribution of genotypes and allele frequencies in the chronic periodontitis and control groups were compared using the χ -test.
RESULTS
The CP group displayed the highest frequency of CT (20%) and TT (6%) genotypes when compared with the control subjects. Allele frequency was found to be similar in both groups. The C allele was found to be predominant in the study population compared with the T allele.
CONCLUSION
The present study denotes that the VDR polymorphism (rs10735810) is not associated with CP in the study group analyzed.
目的
本研究旨在分析维生素D受体（VDR）（rs10735810）基因多态性与慢性牙周炎（CP）之间的关联。
方法
本研究共招募了100名受试者，其中包括50名慢性牙周炎患者和50名健康对照者。从受试者采集的全血中提取基因组DNA。使用位于VDR基因（rs10735810）FokI区域两侧的特异性引物对DNA进行扩增。扩增产物进一步使用FokI酶通过限制性片段长度多态性（RFLP）进行基因分型。记录基于RFLP模式获得的基因型并用于统计分析。使用χ²检验比较慢性牙周炎组和对照组中基因型和等位基因频率的分布。
结果
与对照受试者相比，慢性牙周炎组中CT（20%）和TT（6%）基因型的频率最高。发现两组的等位基因频率相似。与T等位基因相比，研究人群中C等位基因占主导地位。
结论
本研究表明，在所分析的研究组中，VDR多态性（rs10735810）与慢性牙周炎无关。
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