Laboratory of Drug Metabolism and Drug Toxicity, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav St., 1000, Sofia, Bulgaria.
Laboratory of Drug Metabolism and Drug Toxicity, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav St., 1000, Sofia, Bulgaria.
Food Chem Toxicol. 2019 Oct;132:110687. doi: 10.1016/j.fct.2019.110687. Epub 2019 Jul 17.
Muscimol is the main compound found in Amanita muscaria. Several studies have proven that muscimol has suppressive effects on essential tremor, without impairing speech and coordination. The effects of muscimol in Parkinson-affected patients is also described in a number of studies. These studies describe the free radical scavenging and antioxidant activity of the mushroom extract. We have evaluated the possible neuroprotective effects of a standardized extract from A. muscaria, containing high amounts of muscimol, on different models of neurotoxicity in rat brain microsomes, mitochondria, synaptosomes as well as on neuroblastoma cell line SH-SY5Y. The possible inhibitory effect on human recombinant monoaminoxidase-B (hMAOB) enzyme was also studied. The extract revealed statistically significant neuroprotective effects on the in vitro neurotoxicity models and no inhibitory activity on hMAOB.
蝇蕈醇是鹅膏蕈属中主要的化合物。多项研究已经证实蝇蕈醇对原发性震颤有抑制作用，而不会影响言语和协调。多项研究也描述了蝇蕈醇对帕金森病患者的影响。这些研究描述了蘑菇提取物的清除自由基和抗氧化活性。我们评估了一种含有高浓度蝇蕈醇的鹅膏蕈属标准化提取物对大鼠脑微粒体、线粒体、突触体以及神经母细胞瘤 SH-SY5Y 细胞系中不同神经毒性模型的可能神经保护作用。还研究了其对人重组单胺氧化酶-B（hMAOB）酶的可能抑制作用。该提取物对体外神经毒性模型显示出统计学显著的神经保护作用，对 hMAOB 没有抑制活性。
