Department of Electrical Engineering and Computer Science, Graduate School of Engineering and Science, Shibaura Institute of Technology, 3-7-5 Toyosu, Koto-ku, Tokyo 135-8548, Japan.
Department of Electrical Engineering and Computer Science, Graduate School of Engineering and Science, Shibaura Institute of Technology, 3-7-5 Toyosu, Koto-ku, Tokyo 135-8548, Japan.
Food Chem. 2019 Dec 1;300:125189. doi: 10.1016/j.foodchem.2019.125189. Epub 2019 Jul 16.
Since the intake of quercetin glucosides has healthy benefits, the analysis of quercetin glucosides in food is useful. The electrochemical determination of individual quercetin glucosides (quercetin-3-glucoside (Q3G), quercetin-4'-glucoside (Q4'G), and quercetin-3,4'-diglucoside (Q34'G)) in food is carried out. For the detection of quercetin glucosides, a long-length carbon nanotube electrode offers attractive properties such as well-defined current peaks, high sensitivity, and high reproducibility. Cyclic voltammetry (CV) demonstrates distinct and specific peak currents: the oxidation peaks at +0.37, +0.45, and +0.78 V are assigned to the catechol group in the B-ring of Q3G, the 3-hydroxy group in the C-ring of Q4'G, and the resorcinol group in the A-ring of both Q4'G and Q34'G, respectively. Currents, which are determined by CV, of individual quercetin glucosides at the peak potential are proportional to the concentrations of onion, apple peel, and tartary buckwheat, which show good agreement with those obtained by high-performance liquid chromatography.
由于槲皮素葡萄糖苷具有健康益处，因此分析食物中的槲皮素葡萄糖苷很有用。本文对食物中单体槲皮素葡萄糖苷（槲皮素-3-葡萄糖苷（Q3G）、槲皮素-4'-葡萄糖苷（Q4'G）和槲皮素-3,4'-二葡萄糖苷（Q34'G））进行电化学检测。长碳纳米管电极具有明确的电流峰、高灵敏度和高重现性等吸引人的特性，可用于检测槲皮素葡萄糖苷。循环伏安法（CV）显示出独特而特定的峰电流：B 环中邻苯二酚基团的+0.37、+0.45 和+0.78 V 的氧化峰分别归因于 Q3G 中的 Q3G、C 环中 3-羟基基团和 Q4'G 及 Q34'G 中的均苯三酚基团。在峰电位下，通过 CV 确定的单体槲皮素葡萄糖苷的电流与洋葱、苹果皮和苦荞中的浓度成正比，与高效液相色谱法获得的结果吻合良好。
