Animal Infectious Disease Laboratory, School of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, Jiangsu, China; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agri-food Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, Jiangsu, China.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, Jiangsu, China; Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu, China; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agri-food Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, Jiangsu, China.
Virology. 2019 Sep;535:218-226. doi: 10.1016/j.virol.2019.07.001. Epub 2019 Jul 8.
Genotype S H9N2 viruses frequently donate their internal genes to facilitate the generation of novel influenza viruses, e.g., H5N6, H7N9, and H10N8, which have caused human infection. Genotype S was originated from the replacement of F/98-like M and PB2 genes of the genotype H with those from G1-like lineage. However, whether this gene substitution will influence the viral fitness of emerging influenza viruses remains unclear. We found that H5Nx and H7N9 viruses with G1-like PB2 or M gene exhibited higher virulence and replication than those with F/98-like PB2 or M in chickens. We also determined the functional significance of G1-like PB2 in conferring increased polymerase activity and improved nucleus transportation efficiency, and facilitated RNP nuclear export by G1-like M. Our results suggest that G1-like PB2 and M genes optimize viral fitness, and thus play a crucial role in the genesis of emerging influenza viruses that cause rising prevalence in chickens.
基因型 S H9N2 病毒经常通过基因内部交换，从而促进新型流感病毒的产生，例如 H5N6、H7N9 和 H10N8，这些病毒已造成人类感染。基因型 S 起源于 F/98 样 M 和 PB2 基因被来自 G1 样谱系的基因取代。然而，这种基因替换是否会影响新兴流感病毒的病毒适应性仍不清楚。我们发现，具有 G1 样 PB2 或 M 基因的 H5Nx 和 H7N9 病毒在鸡中的毒力和复制能力高于具有 F/98 样 PB2 或 M 的病毒。我们还确定了 G1 样 PB2 在赋予聚合酶活性增加和核运输效率提高，以及 G1 样 M 促进 RNP 核输出方面的功能意义。我们的研究结果表明，G1 样 PB2 和 M 基因优化了病毒适应性，因此在引起鸡中流行率上升的新兴流感病毒的产生中起着至关重要的作用。
