Technical Innovation Service Platform for High Value and High Quality Utilization of Marine Organism, Fuzhou University, Fuzhou 350108, China; Fujian Engineering and Technology Research Center for Comprehensive Utilization of Marine Products Waste, Fuzhou University, Fuzhou 350108, China; Fuzhou Industrial Technology Innovation Center for High Value Utilization of Marine Products, Fuzhou University, Fuzhou 350108, China.
Fujian Engineering and Technology Research Center for Comprehensive Utilization of Marine Products Waste, Fuzhou University, Fuzhou 350108, China; State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.
Bioresour Technol. 2019 Oct;290:121798. doi: 10.1016/j.biortech.2019.121798. Epub 2019 Jul 12.
This study isolated and identified the lutein-enriching microalga Chlorella sorokiniana FZU60. Different types of media and concentrations of sodium acetate and nitrate were evaluated to improve mixotrophic growth and lutein production. Highest lutein content, production, and productivity were obtained in BG11 medium with 1 g/L acetate and 0.75 g/L nitrate. Additionally, pulse feeding with 1 g/L acetate every 48 h led to the alternation between mixotrophy and photoinduction, resulting in a lutein production of 33.6 mg/L. Most notably, excellent lutein content (9.57 mg/g) and productivity (11.57 mg/L/d) were obtained using a new multi-operation integrated strategy, and the achieved levels exceed those reported in most related studies. This work demonstrates the synergistic integration of simple and effective strategies for the enhancement of lutein production in the indigenous microalga C. sorokiniana FZU60 and provides new insight into the highly efficient microalgae-based lutein production.
本研究从富含叶黄素的微藻雨生红球藻中分离并鉴定出叶黄素富集微藻。评估了不同类型的培养基以及不同浓度的乙酸钠和硝酸钠，以提高混养生长和叶黄素的产量。在 BG11 培养基中添加 1g/L 乙酸钠和 0.75g/L 硝酸钠可获得最高的叶黄素含量、产量和生产效率。此外，每隔 48 小时用 1g/L 乙酸钠进行脉冲进料会导致混养和光诱导之间的交替，从而使叶黄素产量达到 33.6mg/L。值得注意的是，使用新的多操作集成策略可获得优异的叶黄素含量（9.57mg/g）和生产效率（11.57mg/L/d），并且所达到的水平超过了大多数相关研究的报道。这项工作展示了简单而有效的策略的协同集成，可用于增强本土微藻雨生红球藻 FZU60 中的叶黄素产量，并为高效基于微藻的叶黄素生产提供了新的见解。
