Bio5 Institute, The University of Arizona, 1657 East Helen Street, PO Box 210240, Tucson, AZ, 85721, USA.
Department of Chemistry, College of Arts and Sciences, Southern Illinois University Edwardsville, 44 Circle Drive, Science Building West, Box - 1652, Edwardsville, IL, 62026-1652, USA.
Environ Sci Pollut Res Int. 2019 Sep;26(26):27435-27443. doi: 10.1007/s11356-019-05960-y. Epub 2019 Jul 20.
Production, distribution, and disposal of pharmaceutical products, including beta-blockers, have become a global issue. Beta-blockers are known to persist in the environment months after their release and may result in the disruption of the homeostatic system in non-target organisms. Here, we study the bioconcentration of three of the most commonly used beta-blockers and their effect on the regeneration of Girardia dorotocephala, a freshwater brown planarian. Acute toxicity tests determined LCs for acebutolol, metoprolol, and propranolol to be 778 mg/L, 711 mg/L, and 111 mg/L, respectively. The quantification and analysis of beta-blocker bioconcentration during acute exposure were performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). After 4 days of exposure to beta-blockers, the bioconcentration drastically decreased for all three beta-blockers at all exposure levels, suggesting that an effective mechanism to reduce uptake or excrete beta-blockers could be present. Additionally, Girardia dorotocephala were cut proximal to the head and the quality of regeneration was documented from each fragment daily. No significant difference was visually observed after 2 weeks of regeneration between the brown planarians placed in beta-blocker solution and those placed in control solution.
药品（包括β受体阻滞剂）的生产、分销和处置已成为全球性问题。β受体阻滞剂在释放数月后仍会在环境中持续存在，并可能导致非靶标生物体内的稳态系统紊乱。在这里，我们研究了三种最常用的β受体阻滞剂的生物浓缩及其对淡水褐蛞蝓（Girardia dorotocephala）再生的影响。急性毒性试验确定了醋丁洛尔、美托洛尔和普萘洛尔的 LC 分别为 778 mg/L、711 mg/L 和 111 mg/L。通过液相色谱-串联质谱法（LC-MS/MS）对急性暴露期间β受体阻滞剂的生物浓缩进行了定量和分析。在暴露于β受体阻滞剂 4 天后，所有三种β受体阻滞剂在所有暴露水平下的生物浓缩都急剧下降，这表明可能存在一种有效的机制来减少β受体阻滞剂的摄取或排泄。此外，将褐蛞蝓靠近头部切断，并每天从每个片段记录再生的质量。在再生 2 周后，与置于对照溶液中的褐蛞蝓相比，置于β受体阻滞剂溶液中的褐蛞蝓在外观上没有观察到明显差异。
