Husni Amir, Lailatussifa Radipta, Isnansetyo Alim
Department of Fisheries Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Sidoarjo Marine and Fisheries Polytechnic, Sidoarjo 61254, Indonesia.
Prev Nutr Food Sci. 2019 Jun;24(2):150-158. doi: 10.3746/pnf.2019.24.2.150. Epub 2019 Jun 30.
This study was conducted to determine the influence of powder (SHP) as an alternative source of functional food for treating stress by measuring levels of glucose, triacylglycerol, total cholesterol, and cortisol, and liver histopathology. Wistar rats aged 3 months and weighing 150~200 g were divided into 7 groups: normal control, fasting control, negative control (stress without adaptogen), and 4 experimental conditions (stress+0.18 mg/kg diazepam, stress+450 mg/kg pellet, stress+mixture of pellet with SHP 450 mg/kg, and stress+450 mg/kg of SHP). Intake of liquids and and body weight were measured daily. Blood samples were collected on day 0 (baseline), day 5, and day 10 to analyze levels of glucose, triacylglycerol, cholesterol, and cortisol. On day 10, rats were euthanized and livers were collected to observe the severity of inflammation. The results indicated that rats receiving SHP 450 mg/kg and the mixture of pellet with SHP 450 mg/kg showed a similar ability as those receiving diazepam 0.18 mg/kg to cope with stress, indicated by an improvement in all blood biochemistry parameters. Supplementation with SHP 450 mg/kg can be used as an alternative source of functional food for overcoming oxidative stress, as indicated by its ability to improve levels of blood glucose, triacylglycerol, total cholesterol, and cortisol, and to improve liver histology by decreasing severity of liver inflammation.
本研究旨在通过测量葡萄糖、三酰甘油、总胆固醇和皮质醇水平以及肝脏组织病理学，来确定粉末状（SHP）作为功能性食品替代来源对治疗应激的影响。将3个月大、体重150~200 g的Wistar大鼠分为7组：正常对照组、禁食对照组、阴性对照组（无适应原的应激组）以及4种实验条件组（应激+0.18 mg/kg地西泮、应激+450 mg/kg颗粒、应激+颗粒与450 mg/kg SHP的混合物、应激+450 mg/kg SHP）。每天测量液体摄入量和体重。在第0天（基线）、第5天和第10天采集血样，以分析葡萄糖、三酰甘油、胆固醇和皮质醇水平。在第10天，对大鼠实施安乐死并采集肝脏，以观察炎症的严重程度。结果表明，接受450 mg/kg SHP以及颗粒与450 mg/kg SHP混合物的大鼠，在应对应激方面表现出与接受0.18 mg/kg地西泮的大鼠相似的能力，所有血液生化参数均有所改善表明了这一点。补充450 mg/kg SHP可作为克服氧化应激的功能性食品替代来源，这体现在其能够改善血糖、三酰甘油、总胆固醇和皮质醇水平，并通过减轻肝脏炎症严重程度来改善肝脏组织学。
