Qiqihar Medical University, Qiqihar, Heilongjiang, P. R. China.
Qiqihar Academy of Medical Sciences, Qiqihar, Heilongjiang, P. R. China.
J Sep Sci. 2019 Oct;42(19):3054-3066. doi: 10.1002/jssc.201900407. Epub 2019 Aug 20.
Corn silk is a well-known traditional Chinese medicine that has been widely used for its antidiabetic, antioxidant, antihyperlipidemic, and other effects in China for thousands of years. Numerous studies have revealed that corn silk contains multiple bioactive constituents that are beneficial for human health. However, the constituents of corn silk in vivo remain ambiguous. In this study, high-throughput ultra-high-performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry technology using multivariate statistical analysis was established to systematically investigate the constituents migrating into blood from corn silk aqueous extract. As a result, 76 compounds were identified, including caffeic acid and ten of its derivatives, (E)-p-coumaric acid and two of its derivatives, ferulic acid and four of its derivatives, and five flavones. Among the identified constituents, 21 constituents, including nine prototype components and 12 metabolites derived from eight components, were characterized in sequence. Based on the significance of the results, the applied approach was powerful for the accurate determination and rapid screening of bioactive components from corn silk aqueous extract. The obtained results are valuable for the in-depth understanding and further pharmacological study of corn silk aqueous extract.
玉米须是一种在中国有着几千年应用历史的传统中药，因其具有降血糖、抗氧化、降血脂等作用而被广泛应用。大量研究表明，玉米须含有多种有益于人体健康的生物活性成分。然而，玉米须在体内的成分仍不明确。本研究采用高通量超高效液相色谱-四级杆飞行时间质谱联用技术结合多元统计分析，系统研究了玉米须水提液中移行入血的成分。结果鉴定出 76 种化合物，包括咖啡酸及其 10 种衍生物、（E）-对香豆酸及其 2 种衍生物、阿魏酸及其 4 种衍生物和 5 种黄酮类化合物。在鉴定出的成分中，有 21 种成分，包括 9 种原型成分和 8 种成分衍生的 12 种代谢产物，依次进行了结构鉴定。基于结果的显著性，该应用方法可用于玉米须水提液中生物活性成分的准确测定和快速筛选。所得结果对深入了解和进一步研究玉米须水提液的药理学具有重要价值。
