Tan Zhenglin, Ma Chunxuan, Xing Xinhui, Zhang Chong
Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
Key Laboratory for Industrial Biocatalysis of the Ministry of Education, Beijing 100084, China.
Sheng Wu Gong Cheng Xue Bao. 2019 Jul 25;35(7):1151-1161. doi: 10.13345/j.cjb.190097.
Microbial cells cultivation is not only the origin, but also the foundation of microbiology. Researches in microbiology can only be carried out when the microbial cells can be cultured. However, conventional microbial cell cultivation is not only time consuming and labour intensive, but human error is also inevitable. Recent years, automated, modularised microbial cells micro-cultivation systems with small volume, good controllability, and equipped with real-time monitoring system have attracted great attention in microbiology. This review presents the state-of-the-art micro-cultivation systems which are implemented in microbial cells cultivation. The key development, applications of various system classified based on their construction, and the prospects of micro-cultivation system are discussed and insights into them are also provided.
微生物细胞培养不仅是微生物学的起源，也是微生物学的基础。只有当微生物细胞能够被培养时，微生物学的研究才能开展。然而，传统的微生物细胞培养不仅耗时费力，而且人为误差也不可避免。近年来，具有体积小、可控性好且配备实时监测系统的自动化、模块化微生物细胞微培养系统在微生物学领域引起了极大关注。本文综述了在微生物细胞培养中应用的最先进的微培养系统。讨论了基于其结构分类的各种系统的关键发展、应用以及微培养系统的前景，并提供了对它们的见解。
