Tang Yonghe, Kong Xiuqi, Xu An, Dong Baoli, Lin Weiying
Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science, University of Jinan, Jinan, Shandong, 250022, P.R. China.
Angew Chem Int Ed Engl. 2016 Mar 1;55(10):3356-9. doi: 10.1002/anie.201510373. Epub 2016 Feb 4.
Investigation of the physiological and pathological functions of formaldehyde (FA) are largely restricted by a lack of useful FA imaging agents, in particular, those that allow detection of FA in the context of living tissues. Herein, we present the rational design, synthesis, and photophysical property studies of the first two-photon fluorescent FA probe, Na-FA. Importantly, the highly desirable attributes of the probe Na-FA (such as a very large turn-on signal (up to 900-fold), a low detection limit, and a very fast onset imparted by the unique design aspects of the probe), make it possible to monitor endogenous FA in living tissues for the first time. Furthermore, sodium bisulfite was identified as a simple and convenient inhibitor of FA within biological environments.
由于缺乏有效的甲醛（FA）成像剂，尤其是那些能够在活体组织中检测FA的成像剂，对FA生理和病理功能的研究在很大程度上受到限制。在此，我们展示了首个双光子荧光FA探针Na-FA的合理设计、合成及光物理性质研究。重要的是，探针Na-FA具有非常理想的特性（如非常大的开启信号（高达900倍）、低检测限以及由探针独特设计带来的非常快速的响应），首次使得在活体组织中监测内源性FA成为可能。此外，亚硫酸氢钠被确定为生物环境中一种简单便捷的FA抑制剂。
