Li Guili, Sun Shuhong, Zhao Zhong Yuan, Sun Yunfang
Department of Clinical Laboratory, Affiliated Hospital of Shandong Medical College, Shandong, China.
Department of Clinical Laboratory, Liny People's Hospital, Shandong, China.
J Int Med Res. 2019 Sep;47(9):4344-4352. doi: 10.1177/0300060519863544. Epub 2019 Jul 21.
To investigate the pathogenicity of (KPN) possessing or the aerobactin gene in infected mice.
BALB/c mice were divided into four groups (n = 10 per group) and infected with: string test-positive and -positive or aerobactin-positive KPN (group 1), string test-negative but -positive KPN (group 2), string test-negative but aerobactin-positive KPN (group 3), or string test- and /aerobactin-negative KPN (group 4). Mouse survival time was compared among groups, and the infection of livers, spleens, lungs, and kidneys and KPN growth were assessed in infected mice.
Compared with the negative group (group 4), the survival rates of mice infected with - or aerobactin-positive KPN (groups 1–3) were significantly lower, their multi-organ injuries were significantly more severe, and bacterial enumeration was significantly higher.
Despite being string test-negative, aerobactin- or -positive KPN still exhibit high virulence and anti-immune effect activity. Therefore, the combination of the string test and gene detection of aerobactin and will be helpful in screening hypervirulent KPN.
研究携带或气杆菌素基因的肺炎克雷伯菌（KPN）在感染小鼠中的致病性。
将BALB/c小鼠分为四组（每组n = 10），并分别感染：悬滴试验阳性且或气杆菌素阳性的KPN（第1组）、悬滴试验阴性但阳性的KPN（第2组）、悬滴试验阴性但气杆菌素阳性的KPN（第3组）或悬滴试验及/和气杆菌素阴性的KPN（第4组）。比较各组小鼠的生存时间，并评估感染小鼠的肝脏、脾脏、肺和肾脏感染情况及KPN生长情况。
与阴性组（第4组）相比，感染或气杆菌素阳性KPN的小鼠（第1 - 3组）存活率显著降低，多器官损伤显著更严重，细菌计数显著更高。
尽管悬滴试验阴性，但气杆菌素或阳性的KPN仍表现出高毒力和抗免疫效应活性。因此，悬滴试验与气杆菌素和基因检测相结合将有助于筛选高毒力KPN。
