Kot Barbara, Witeska Małgorzata, Szweda Piotr, Piechota Małgorzata, Kondera Elżbieta, Horoszewicz Elżbieta, Balak Izabela, Bin Hafeez Ahmer, Synowiec Alicja
Institute of Biological Sciences, Faculty of Exact and Natural Sciences, University of Siedlce, 14 Bolesława Prusa Str., 08-110 Siedlce, Poland.
Department of Animal Environment Biology, Institute of Animal Science, Warsaw University of Life Sciences-SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland.
Pathogens. 2025 Jun 30;14(7):648. doi: 10.3390/pathogens14070648.
In hospital environments, pathogenic bacteria spread easily and acquire virulence and antibiotic resistance genes. The aim of the study was an evaluation of the genetic diversity of 109 isolates recovered from patients of a district hospital in central Poland. The frequencies of genes coding for β-lactamases, efflux pumps, and virulence factors were determined. Genotyping of the isolates was performed with ERIC (Enterobacterial Repetitive Intergenic Consensus) and REP (Repetitive Element Sequence Based) PCR techniques, with 21 and 19 genotypes being identified, respectively. The (92.7%) group 1 (83.5%), (28.4%), (16.5%) (11.0%), group 9 (3.7%), (1.8%), , , group 2, groups 8, and 25/26 (0% each) and efflux pumps: (100%) (93.6%) and (60.5%), and virulence genes coding: urease subunit (94.5%) endotoxins (92.7%) and (64.2%), and siderophore (3.7%) were detected. The , group 1, , , (16.5%); , group 1, , (15.6%), and , group 1, , , , (11.9%) were the most common resistance patterns. The distribution of resistance and virulence genes varied more between hospital wards than between different clinical materials. Hospital's antibiotic-resistant and virulent , able to spread among humans, animals, and in the environment, pose a significant threat to public health.
在医院环境中，病原菌容易传播并获得毒力和抗生素抗性基因。本研究的目的是评估从波兰中部一家地区医院的患者中分离出的109株菌株的遗传多样性。测定了编码β-内酰胺酶、外排泵和毒力因子的基因频率。采用ERIC（肠杆菌重复基因间共有序列）和REP（基于重复元件序列）PCR技术对分离株进行基因分型，分别鉴定出21种和19种基因型。检测到β-内酰胺酶基因：第1组（92.7%）、第83.5组（83.5%）、第28.4组（28.4%）、第16.5组（16.5%）、第11.0组（11.0%）、第9组（3.7%）、第1.8组（1.8%）、第2组、第8组和第25/26组（每组0%）；外排泵基因：第100组（100%）、第93.6组（93.6%）和第60.5组（60.5%）；以及编码毒力基因：脲酶亚基（94.5%）、内毒素（92.7%）和第64.2组（64.2%），以及铁载体（3.7%）。第16.5组、第1组、第15.6组、第11.9组（16.5%）；第15.6组、第1组、第11.9组（15.6%），以及第11.9组、第1组、第16.5组（11.9%）是最常见的耐药模式。耐药和毒力基因的分布在医院病房之间的差异大于不同临床材料之间的差异。医院中具有抗生素抗性和毒力的菌株，能够在人类、动物和环境中传播，对公众健康构成重大威胁。
