Grześkowiak Łukasz, Riedmüller Jonathan, Vahjen Wilfried, Zentek Jürgen
Institute of Animal Nutrition, Freie Universität Berlin, Berlin, Germany.
J Vet Diagn Invest. 2020 Mar;32(2):222-225. doi: 10.1177/1040638719864383. Epub 2019 Jul 22.
Storage procedures are known to affect the detectability of toxins in equine and human feces. We assessed the impact of different storage conditions on the detectability of toxins in swine feces. Specimens were inoculated with toxins, 112 ng/g of toxin A (TcdA) and 16 ng/g of toxin B (TcdB) and subjected to the following 3 storage treatments: 4°C, -30°C, repetitive freezing at -30°C and thawing. Toxin determination was assessed at 1, 2, 7, 14, and 21 d with ELISA. A decrease in concentrations of TcdA with time was observed for samples stored at 4°C and repetitive freezing-thawing ( ≤0.05). On day 14, storage at 4°C resulted in decreased TcdA concentration as opposed to storage at -30°C and repetitive freezing-thawing ( ≤0.05). On day 21, storage at 4°C resulted in decreased TcdA detectability compared with storage at -30°C ( ≤0.05). The TcdB concentration was unaffected. These results on toxin detectability in swine feces should be carefully considered in in vitro studies on toxigenic Our results also offer valuable information for microbiologists and veterinarians monitoring the presence of virulent in pigs.
已知储存程序会影响马和人类粪便中毒素的可检测性。我们评估了不同储存条件对猪粪便中毒素可检测性的影响。将样本接种毒素，毒素A（TcdA）为112 ng/g，毒素B（TcdB）为16 ng/g，并进行以下3种储存处理：4°C、-30°C、在-30°C反复冻融。在第1、2、7、14和21天用酶联免疫吸附测定法（ELISA）评估毒素测定情况。对于储存在4°C和反复冻融的样本，观察到TcdA浓度随时间下降（P≤0.05）。在第14天，与储存在-30°C和反复冻融相比，储存在4°C导致TcdA浓度下降（P≤0.05）。在第21天，与储存在-30°C相比，储存在4°C导致TcdA可检测性下降（P≤0.05）。TcdB浓度未受影响。在关于产毒[相关内容]的体外研究中，应仔细考虑这些关于猪粪便中毒素可检测性的结果。我们的结果也为微生物学家和兽医监测猪体内致病[相关内容]的存在提供了有价值的信息。
